Chicken anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 594)

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Antigen
Epitope
Heavy & Light Chain
4446
2573
1789
1039
928
825
305
272
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Goat
2685
2654
2280
1644
1386
741
607
423
364
338
330
324
307
258
168
148
126
114
41
27
25
22
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Chicken
6579
4500
1779
792
636
372
87
52
50
9
8
5
5
4
2
1
1
Conjugate
DyLight 594
2125
1808
1666
1315
717
630
521
297
292
284
274
268
217
186
177
117
102
79
69
58
54
40
35
26
23
22
19
19
19
19
19
16
15
15
15
15
15
15
14
13
12
11
11
10
10
9
9
9
9
9
9
9
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified Goat IgG, whole molecule
Cross-Reactivity (Details) min x/human, mouse or rabbit IgG, serum proteins
Characteristics Chicken anti-Goat IgG (H&L) - Affinity Pure, DyLight 594 Conjugate.
Fluorphore: DyLight 594 (Ex = 593 nm, Em = 618 nm).
Fluor Protein Ratio: Moles DyLight 594 per Mole Antibody.
Purification Affinity purified using solid phase mouse IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Chicken Ig fraction was prepared using eggs from healthy hens of US origin.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C
Product cited in: Jin, Zhu, Sun, Mao, Xie, Greenberg: "Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 18, pp. 11946-50, 2002 (PubMed).

Kernie, Erwin, Parada: "Brain remodeling due to neuronal and astrocytic proliferation after controlled cortical injury in mice." in: Journal of neuroscience research, Vol. 66, Issue 3, pp. 317-26, 2001 (PubMed).

Steinert, Chou, Prahlad, Parry, Marekov, Wu, Jang, Goldman: "A high molecular weight intermediate filament-associated protein in BHK-21 cells is nestin, a type VI intermediate filament protein. Limited co-assembly in vitro to form heteropolymers with type III vimentin and type IV alpha-internexin." in: The Journal of biological chemistry, Vol. 274, Issue 14, pp. 9881-90, 1999 (PubMed).