Chicken anti-Goat IgG (H&L) Antibody

Antigen: IgG

Epitope: H&L

Host: Chicken

Reactivity: Goat

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN611661

Additional Information

Alternative name: IgG (H&L)

Immunogen: Purified Goat IgG, whole molecule

Format: Lyophilized

Description: Chicken anti-Goat IgG (H&L) - Affinity Pure, DyLight 650 Conjugate, min x/human, mouse or rabbit IgG, serum proteins. Country of Origin: Chicken Ig fraction was prepared using eggs from healthy hens of US origin. Fluorphore: DyLight 650 (Ex = 652 nm, Em = 672 nm). Fluor Protein Ratio: Moles DyLight 650 per Mole Antibody.

Comments:

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Concentration: 1 mg/ml (E 1% at 280 nm = 13.2)

Purity: Affinity purified antibody is > 95% based on SDS-PAGE

Purification: Affinity purified using solid phase goat IgG (H&L)

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide

Storage: Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 0.55 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.

Research Area: Secondary Antibodies

Restrictions: For Research Use only