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Chicken anti-Rat (Rattus) IgG (Heavy & Light Chain) Antibody

Antigen

IgG

Binding Site
Alternatives

Heavy & Light Chain

Host
Alternatives

Chicken

Reactivity
Alternatives

Rat (Rattus)

Conjugate
Alternatives Un-conjugated
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
3 references available
Catalog no. ABIN611706
Quantity 1 mg  (1 mg/ml (E 1% at 280 nm = 13.2))  (Variants)
Price 190.14 $   Plus shipping costs $45.00
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Additional Information

Immunogen Purified Rat IgG, whole molecule
Format Lyophilized
Description Chicken anti-Rat IgG (H&L) - Affinity Pure, DyLight 650 Conjugate. Country of Origin: Chicken Ig fraction was prepared using eggs from healthy hens of US origin. Fluorphore: DyLight 650 (Ex = 652 nm, Em = 672 nm). Fluor Protein Ratio: Moles DyLight 650 per Mole Antibody.
Comments

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Application Details

Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications
Concentration 1 mg/ml (E 1% at 280 nm = 13.2)
Purity Affinity purified antibody is > 95% based on SDS-PAGE
Purification Affinity purified using solid phase goat IgG (H&L)
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide
Storage Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.
Research Area Secondary Antibodies
Restrictions For Research Use only

Publications

Product des Portes, Pinard, Billuart et al.: "A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome." in: Cell, Vol. 92, Issue 1, pp. 51-61, 1998 (PubMed).

Gleeson, Allen, Fox et al.: "Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein." in: Cell, Vol. 92, Issue 1, pp. 63-72, 1998 (PubMed).

Magavi, Leavitt, Macklis: "Induction of neurogenesis in the neocortex of adult mice." in: Nature, Vol. 405, Issue 6789, pp. 951-5, 2000 (PubMed).

Alternatives

Alternatives for antigen "IgG", type "Secondary Antibodies"
Hosts Goat (3758), Rabbit (2825), Donkey (1023), Chicken (370), Mouse (291), Sheep (199), Guinea Pig (29), Rat (20), Pig (14), Duck (6), Horse (5), Cat (Feline) (2), Hamster (1), Human (1)
Reactivities Mouse (Murine) (1409), Human (1361), Rabbit (1142), Rat (Rattus) (948), Goat (707), Guinea Pig (388), Sheep (Ovine) (343), Cow (Bovine) (273), Cat (Feline) (202), Dog (Canine) (197), Horse (Equine) (188), Pig (Porcine) (185), Chicken (172), Hamster (128), Donkey (75), Monkey (70), Golden Syrian Hamster (69), Syrian Hamster (62), Llama (51), Armenian Hamster (27), Ferret (19), Mink (18), Turkey (13), Bird (Avian) (6), Rhesus Monkey (5), Deer (4), Duck (3), Pigeon (3), mouse (Murine) (1)
Conjugates HRP (1238), FITC (1159), Biotin (985), Alkaline Phosphatase (AP) (859), Texas Red (TR) (568), TRITC (376), Rhodamine (369), DyLight 594 (199), DyLight 800 (186), PE (141), DyLight 650 (116), DyLight 633 (115), DyLight 550 (113), DyLight 350 (96), RPE (64), Cy3 (39), Cy5 (39), Gold (37), APC (34), Alexa Fluor 647 (34), Alexa Fluor 350 (33), Alexa Fluor 488 (33), Alexa Fluor 555 (33), Cy5.5 (33), Cy7 (33), PE-Cy3 (33), PE-Cy5 (33), PE-Cy5.5 (33), PE-Cy7 (33), Agarose Beads (27), DyLight 488 (20), SureLight® R-PE (15), DyLight 405 (13), DyLight 680 (13), Atto 425 (10), Atto 488 (10), Atto 532 (10), Atto 550 (10), Atto 594 (10), Atto 647N (10), Atto 655 (10), Colloidal Gold (10nm) (8), Colloidal Gold (5nm) (7), SPRD (7), Beads (6), Peroxidase (POD) (6), Poly-HRP40 (4), beta-Gal (4), Poly-HRP (3), PromoFluor-350 (3), PromoFluor-405 (3), PromoFluor-415 (3), PromoFluor-488 Premium (3), PromoFluor-488LSS (3), PromoFluor-500LSS (3), PromoFluor-505 (3), PromoFluor-510LSS (3), PromoFluor-514LSS (3), PromoFluor-520LSS (3), PromoFluor-532 (3), PromoFluor-546 (3), PromoFluor-555 (3), PromoFluor-590 (3), PromoFluor-610 (3), PromoFluor-633 (3), PromoFluor-647 (3), PromoFluor-670 (3), PromoFluor-680 (3), PromoFluor-700 (3), PromoFluor-750 (3), PromoFluor-770 (3), PromoFluor-780 (3), PromoFluor-840 (3), AMCA (2), Chromeo™ 488 (2), Chromeo™ 546 (2), Colloidal Gold (20nm) (2), SureLight® P3 (2), Chromeo™ 494 (1), Chromeo™ 642 (1), Colloidal Gold (15nm) (1), DTAF (1), FL (1), PE,Cy7 (1), PE,RPE (1), Poly-HRP80 (1)
Epitopes Heavy & Light Chain (3818), Fc Region (766), F(ab')2 Region (649), Fc Region, Chain gamma (78), Heavy Chain (54), Chain gamma (47), H&L (45), Fab Region (39), Chain gamma 1,Chain gamma 2b,Chain gamma 2a,Chain gamma 3 (14), Light Chain (7), Chain gamma,Fc Region (4), N-Term (2), Native Conformation (2), Chain alpha (1), Heavy Chain, Fc Region (1)