You are viewing an incomplete version of our website. Please click to reload the website as full version.

Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 594)

Details for Product No. ABIN611731, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4526
1866
1786
962
643
280
279
126
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Goat
2473
2411
2069
1552
1291
650
565
392
333
310
310
304
295
217
159
132
107
78
32
24
21
13
12
8
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
Host
Donkey
6122
4267
1526
652
566
300
85
49
45
8
8
4
4
3
1
1
Conjugate
DyLight 594
1928
1760
1562
1137
606
530
523
274
202
183
179
172
166
166
138
79
69
47
47
38
37
23
22
20
19
19
15
14
14
14
14
14
13
11
11
10
10
10
10
10
10
9
9
9
9
9
9
8
8
8
8
8
8
8
7
7
6
6
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen Purified Goat IgG, whole molecule
Characteristics Donkey anti-Goat IgG (H&L) - Affinity Pure, DyLight 594 Conjugate.
Fluorphore: DyLight 594 (Ex = 593 nm, Em = 618 nm).
Fluor Protein Ratio: Moles DyLight 594 per Mole Antibody.
Purification Affinity purified using solid phase mouse IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C
Product cited in: Martinez-Exposito, Kaplan, Copeland, Sorger: "Retention of the BUB3 checkpoint protein on lagging chromosomes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 15, pp. 8493-8, 1999

Taylor, Ha, McKeon: "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase." in: The Journal of cell biology, Vol. 142, Issue 1, pp. 1-11, 1998