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Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (DyLight 550)

Details for Product No. ABIN611820, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4489
1723
1694
896
804
618
560
280
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Rabbit
2711
2631
2318
1653
1396
733
609
422
365
336
332
327
311
259
165
148
124
112
41
27
21
21
12
11
7
5
5
5
4
4
4
4
4
4
4
4
4
3
3
2
2
1
1
1
1
Host
Donkey
6625
4577
1762
767
650
367
97
51
50
9
8
5
4
3
1
1
Conjugate
DyLight 550
2146
1833
1702
1312
720
633
528
301
296
284
274
266
216
187
178
117
101
79
69
55
54
40
35
25
23
21
19
18
15
15
15
15
14
14
14
14
13
11
10
10
10
10
10
10
9
9
9
9
9
8
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified Rabbit IgG, whole molecule
Fragment F(ab')2 fragment
Characteristics Donkey anti-Rabbit IgG (H&L) - F(ab) '2 fragment, DyLight 550 Conjugate.
Fluorphore: DyLight 550 (Ex = 550 nm, Em = 576 nm).
Fluor Protein Ratio: Moles DyLight 550 per Mole Antibody.
Purification Affinity purified using solid phase rat IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Product cited in: Mikita, Campbell, Wu, Williamson, Schindler: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, 1996 (PubMed).

Hou, Schindler, Henzel, Ho, Brasseur, McKnight: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, 1994 (PubMed).

Background publications Quelle, Shimoda, Thierfelder, Fischer, Kim, Ruben, Cleveland, Pierce, Keegan, Nelms: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." in: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, 1995 (PubMed).