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Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody
Heavy & Light Chain
Flow Cytometry (FACS), Immunofluorescence (IF)
|3 references available|
|Quantity||0.5 mg (1 mg/ml (E 1% at 280 nm = 13.0)) (Variants)|
|Price||155.57 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||Purified Rabbit IgG, whole molecule|
|Description||Donkey anti-Rabbit IgG (H&L) - F(ab)'2 fragment, DyLight 550 Conjugate. Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 550 (Ex = 550 nm, Em = 576 nm). Fluor Protein Ratio: Moles DyLight 550 per Mole Antibody.|
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
|Application Notes||This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications|
|Concentration||1 mg/ml (E 1% at 280 nm = 13.0)|
|Purity||Affinity purified antibody is > 95% based on SDS-PAGE|
|Purification||Affinity purified using solid phase rat IgG (H&L)|
|Buffer||10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide|
|Storage||Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 0.55 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.|
|Research Area||Secondary Antibodies|
|Restrictions||For Research Use only|
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Hou, Schindler, Henzel et al.: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, 1994 (PubMed).
Mikita, Campbell, Wu et al.: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, 1996 (PubMed).