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Details for Product No. ABIN611821

Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (DyLight 594)

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Antigen
Epitope
»Alternatives Heavy & Light Chain
Reactivity
»Alternatives Rabbit
Host
»Alternatives Donkey
Conjugate
»Alternatives DyLight 594
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
Pubmed 3 references available
Catalog no. ABIN611821
Quantity 0.5 mg
Price
190.14 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen Purified Rabbit IgG, whole molecule
Fragment F(ab')2 fragment
Purification Affinity purified using solid phase rat IgG (H&L)
Purity Affinity purified antibody is > 95% based on SDS-PAGE
Background Donkey anti-Rabbit IgG (H&L) - F(ab)'2 fragment, DyLight 594 Conjugate. Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 594 (Ex = 593 nm, Em = 618 nm). Fluor Protein Ratio: Moles DyLight 594 per Mole Antibody.
Research Area Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications
Comment

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/ml (E 1% at 280 nm = 13.0)
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide
Preservative Sodium azide
Storage 4 °C
Product cited in: Quelle, Shimoda, Thierfelder et al.: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." in: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, 1995 (PubMed).

Hou, Schindler, Henzel et al.: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, 1994 (PubMed).

Mikita, Campbell, Wu et al.: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, 1996 (PubMed).

Alternatives for antigen "IgG", type "Secondary Antibodies"
Hosts (3909), (2738), (1026), (379), (209), (208), (29), (26), (14), (6), (3), (3), (2), (1), (1)
Reactivities (1575), (1385), (1281), (1023), (786), (406), (351), (229), (227), (204), (188), (184), (179), (175), (89), (76), (62), (53), (32), (31), (18), (12), (6), (5), (4), (3), (3), (1)
Conjugates (1311), (1201), (1020), (853), (582), (412), (367), (201), (198), (116), (115), (113), (111), (101), (84), (31), (27), (20), (20), (17), (15), (13), (13), (10), (10), (10), (10), (10), (10), (10), (7), (7), (6), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4077), (941), (683), (143), (67), (65), (40), (20), (14), (14), (14), (14), (4), (4), (2), (1)
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