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Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (DyLight 594)

Details for Product No. ABIN611821, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4392
2530
1765
1035
925
824
306
283
35
34
31
8
6
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Rabbit
2657
2627
2286
1636
1388
738
607
422
365
337
333
322
310
259
165
147
124
112
41
27
25
21
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Donkey
6523
4540
1761
768
639
365
93
51
50
9
8
5
4
3
1
1
Conjugate
DyLight 594
2119
1814
1663
1306
717
630
532
296
293
284
274
267
216
187
177
117
101
79
69
55
54
40
35
25
23
22
19
19
19
19
18
15
15
15
15
15
15
15
14
13
12
10
10
10
9
9
9
9
9
8
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified Rabbit IgG, whole molecule
Fragment F(ab')2 fragment
Characteristics Donkey anti-Rabbit IgG (H&L) - F(ab) '2 fragment, DyLight 594 Conjugate.
Fluorphore: DyLight 594 (Ex = 593 nm, Em = 618 nm).
Fluor Protein Ratio: Moles DyLight 594 per Mole Antibody.
Purification Affinity purified using solid phase rat IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C
Product cited in: Mikita, Campbell, Wu, Williamson, Schindler: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, 1996 (PubMed).

Hou, Schindler, Henzel, Ho, Brasseur, McKnight: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, 1994 (PubMed).

Background publications Quelle, Shimoda, Thierfelder, Fischer, Kim, Ruben, Cleveland, Pierce, Keegan, Nelms: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." in: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, 1995 (PubMed).