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Donkey anti-Sheep (Ovine) IgG (Heavy & Light Chain) Antibody

Details for Product No. ABIN611882, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4517
1867
1732
962
643
277
203
56
35
34
31
8
8
5
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Sheep (Ovine)
2468
2360
2055
1545
1279
627
581
401
328
315
315
309
293
224
164
112
105
76
32
24
21
13
12
8
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
Host
Donkey
6100
4268
1526
605
545
298
85
49
43
8
8
4
4
3
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
Supplier
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Immunogen Purified Sheep IgG, whole molecule
Fragment F(ab')2 fragment
Characteristics Donkey anti-Sheep IgG (H&L) - F(ab) '2 fragment, DyLight 650 Conjugate.
Fluorphore: DyLight 650 (Ex = 652 nm, Em = 672 nm).
Fluor Protein Ratio: Moles DyLight 650 per Mole Antibody.
Purification Affinity purified using solid phase cat IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Product cited in: Fallon, Moreau, Croft et al.: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, 2002 (PubMed).

Sugita, Khvochtev, Südhof: "Neurexins are functional alpha-latrotoxin receptors." in: Neuron, Vol. 22, Issue 3, pp. 489-96, 1999 (PubMed).

Missler, Südhof: "Neurexins: three genes and 1001 products." in: Trends in genetics : TIG, Vol. 14, Issue 1, pp. 20-6, 1998 (PubMed).

Ushkaryov, Petrenko, Geppert et al.: "Neurexins: synaptic cell surface proteins related to the alpha-latrotoxin receptor and laminin." in: Science (New York, N.Y.), Vol. 257, Issue 5066, pp. 50-6, 1992 (PubMed).