Donkey anti-Sheep (Ovine) IgG (Heavy & Light Chain) Antibody

Antigen: IgG

Binding Site: Heavy & Light Chain

Host: Donkey

Reactivity: Sheep (Ovine)

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN611882

Additional Information

Fragment: F(ab')2 fragment

Immunogen: Purified Sheep IgG, whole molecule

Format: Lyophilized

Description: Donkey anti-Sheep IgG (H&L) - F(ab)'2 fragment, DyLight 650 Conjugate. Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 650 (Ex = 652 nm, Em = 672 nm). Fluor Protein Ratio: Moles DyLight 650 per Mole Antibody.

Comments:

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Concentration: 1 mg/ml (E 1% at 280 nm = 13.0)

Purity: Affinity purified antibody is > 95% based on SDS-PAGE

Purification: Affinity purified using solid phase cat IgG (H&L)

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide

Storage: Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 0.55 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.

Research Area: Secondary Antibodies

Restrictions: For Research Use only

Publications

Product: Ushkaryov, Petrenko, Geppert et al.: "Neurexins: synaptic cell surface proteins related to the alpha-latrotoxin receptor and laminin." in: Science (New York, N.Y.), Vol. 257, Issue 5066, pp. 50-6, 1992 (PubMed).

Missler, Südhof: "Neurexins: three genes and 1001 products." in: Trends in genetics : TIG, Vol. 14, Issue 1, pp. 20-6, 1998 (PubMed).

Sugita, Khvochtev, Südhof: "Neurexins are functional alpha-latrotoxin receptors." in: Neuron, Vol. 22, Issue 3, pp. 489-96, 1999 (PubMed).

Fallon, Moreau, Croft et al.: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, 2002 (PubMed).