Donkey anti-Sheep (Ovine) IgG (Heavy & Light Chain) Antibody

Details for Product No. ABIN611882
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Antigen
Epitope
Heavy & Light Chain
(3560), (2017), (1427), (1035), (763), (229), (189), (73), (33), (32), (29), (7), (5), (2), (2), (2), (2), (1), (1)
Reactivity
Sheep (Ovine)
(1891), (1869), (1545), (1214), (972), (496), (435), (343), (297), (266), (266), (260), (225), (222), (121), (93), (85), (81), (32), (28), (21), (14), (8), (8), (7), (5), (5), (4), (3), (3), (2), (1), (1), (1)
Host
Donkey
(4790), (3558), (1181), (463), (395), (254), (67), (44), (25), (8), (5), (1)
Conjugate
Un-conjugated
(1511), (1380), (1248), (1003), (674), (553), (413), (201), (199), (116), (115), (113), (111), (107), (100), (33), (32), (21), (20), (15), (11), (10), (10), (10), (10), (10), (10), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
Pubmed 4 references available
Quantity 0.5 mg
Options
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Catalog No. ABIN611882
190.14 $
Plus shipping costs $45.00

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  • +1 404 474 4654
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Immunogen Purified Sheep IgG, whole molecule
Fragment F(ab')2 fragment
Characteristics Donkey anti-Sheep IgG (H&L) - F(ab) '2 fragment, DyLight 650 Conjugate.
Fluorphore: DyLight 650 (Ex = 652 nm, Em = 672 nm).
Fluor Protein Ratio: Moles DyLight 650 per Mole Antibody.
Purification Affinity purified using solid phase cat IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Product cited in: Ushkaryov, Petrenko, Geppert et al.: "Neurexins: synaptic cell surface proteins related to the alpha-latrotoxin receptor and laminin." in: Science (New York, N.Y.), Vol. 257, Issue 5066, pp. 50-6, 1992 (PubMed).

Missler, Südhof: "Neurexins: three genes and 1001 products." in: Trends in genetics : TIG, Vol. 14, Issue 1, pp. 20-6, 1998 (PubMed).

Sugita, Khvochtev, Südhof: "Neurexins are functional alpha-latrotoxin receptors." in: Neuron, Vol. 22, Issue 3, pp. 489-96, 1999 (PubMed).

Fallon, Moreau, Croft et al.: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, 2002 (PubMed).

Validation Images
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