Goat anti-Human IgG (Fc Region, Chain gamma) Antibody

Antigen: IgG

Binding Site: Fc Region, Chain gamma

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN612054

Additional Information

Immunogen: Purified human IgG Fc, (gamma chain)

Format: Lyophilized

Description: Goat anti-human IgG Fc (gamma chain) - Affinity Pure, DyLight 350 Conjugate. Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 350 (Ex = 353 nm, Em = 432 nm). Fluor Protein Ratio: Moles DyLight 350 per Mole Antibody.

Comments:

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

Purity: Affinity purified antibody is > 95% based on SDS-PAGE

Purification: Affinity purified using solid phase rabbit IgG (H&L)

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide

Storage: Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.

Restrictions: For Research Use only

Publications

Product: Saluja, Vassallo, Tanese: "Distinct subdomains of human TAFII130 are required for interactions with glutamine-rich transcriptional activators." in: Molecular and cellular biology, Vol. 18, Issue 10, pp. 5734-43, 1998 (PubMed).

Shimohata, Nakajima, Yamada et al.: "Expanded polyglutamine stretches interact with TAFII130, interfering with CREB-dependent transcription." in: Nature genetics, Vol. 26, Issue 1, pp. 29-36, 2000 (PubMed).

Freiman, Albright, Chu et al.: "Redundant role of tissue-selective TAF(II)105 in B lymphocytes." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6564-72, 2002 (PubMed).