Goat anti-Human IgG (Fc Region), (Chain gamma) Antibody (DyLight 350)

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Antigen
Epitope
Fc Region, Chain gamma
4447
2573
1788
1039
928
825
304
272
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
2685
2653
2280
1644
1387
741
607
423
364
338
330
324
307
258
168
148
126
114
41
27
25
22
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Goat
6578
4500
1779
792
637
372
87
52
50
9
8
5
5
4
2
1
1
Conjugate
DyLight 350
2125
1808
1666
1315
717
630
521
297
292
284
274
268
216
186
178
117
102
79
69
58
54
40
35
26
23
22
19
19
19
19
19
16
15
15
15
15
15
15
14
13
12
11
11
10
10
9
9
9
9
9
9
9
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified human IgG Fc, (gamma chain)
Characteristics Fluorphore: DyLight 350 (Ex = 353 nm, Em = 432 nm).
Fluor Protein Ratio: Moles DyLight 350 per Mole Antibody.
Purification Affinity purified using solid phase rabbit IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

Restrictions For Research Use only
Format Lyophilized
Concentration 1.0 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Product cited in: Freiman, Albright, Chu, Zheng, Liang, Sha, Tjian: "Redundant role of tissue-selective TAF(II)105 in B lymphocytes." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6564-72, 2002 (PubMed).

Shimohata, Nakajima, Yamada, Uchida, Onodera, Naruse, Kimura, Koide, Nozaki, Sano, Ishiguro, Sakoe, Ooshima, Sato, Ikeuchi, Oyake, Sato, Aoyagi, Hozumi, Nagatsu, Takiyama, Nishizawa, Goto, Kanazawa et al.: "Expanded polyglutamine stretches interact with TAFII130, interfering with CREB-dependent transcription. ..." in: Nature genetics, Vol. 26, Issue 1, pp. 29-36, 2000 (PubMed).

Saluja, Vassallo, Tanese: "Distinct subdomains of human TAFII130 are required for interactions with glutamine-rich transcriptional activators." in: Molecular and cellular biology, Vol. 18, Issue 10, pp. 5734-43, 1998 (PubMed).