Goat anti-Human IgM (Chain mu) Antibody (DyLight 800)

Details for Product No. ABIN612152
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Antigen
Epitope
Chain mu
(605), (253), (127), (60), (45), (22), (15), (5), (4), (2), (1), (1), (1)
Reactivity
Human
(751), (366), (173), (61), (60), (43), (38), (37), (32), (30), (28), (24), (24), (14), (6), (5), (4), (4), (1)
Host
Goat
(859), (373), (292), (114), (23), (9), (8), (5), (2)
Conjugate
DyLight 800
(239), (219), (176), (110), (55), (55), (43), (39), (24), (19), (16), (16), (11), (11), (11), (11), (6), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
Pubmed 4 references available
Quantity 0.5 mg
Options
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Catalog No. ABIN612152
237.29 $
Plus shipping costs $45.00

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Immunogen Purified human IgM (mu chain)
Fragment F(ab')2 fragment
Characteristics Goat anti-human IgM ( chain) - F(ab)2 fragment, DyLight 800 Conjugate.
Fluorphore: DyLight 800 (Ex = 777 nm, Em = 794 nm).
Fluor Protein Ratio: Moles DyLight 800 per Mole Antibody.
Purification Affinity purified using solid phase human IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1.0 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C
Product cited in: Grundhoff, Kremmer, Türeci et al.: "Characterization of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C." in: The Journal of biological chemistry, Vol. 274, Issue 27, pp. 19136-44, 1999 (PubMed).

Charroux, Pellizzoni, Perkinson et al.: "Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems." in: The Journal of cell biology, Vol. 147, Issue 6, pp. 1181-94, 2000 (PubMed).

Meister, Bühler, Laggerbauer et al.: "Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins." in: Human molecular genetics, Vol. 9, Issue 13, pp. 1977-86, 2000 (PubMed).

Ou, Mouillet, Yan et al.: "The DEAD box protein DP103 is a regulator of steroidogenic factor-1." in: Molecular endocrinology (Baltimore, Md.), Vol. 15, Issue 1, pp. 69-79, 2001 (PubMed).

Validation Images
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