Goat anti-Human IgM (Chain mu) Antibody

Antigen: IgM

Binding Site: Chain mu

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN612153

Additional Information

Immunogen: Purified human IgM ( chain)

Format: Lyophilized

Description: Goat anti-human IgM ( chain) - F(ab) 2 fragment, DyLight 350 Conjugate, min x w/human IgA + IgG. Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 350 (Ex = 353 nm, Em = 432 nm). Fluor Protein Ratio: Moles DyLight 350 per Mole Antibody.

Comments:

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

Purity: Affinity purified antibody is > 95% based on SDS-PAGE

Purification: Affinity purified using solid phase human IgM (H&L)

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide

Storage: Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.

Restrictions: For Research Use only

Publications

Product: Grundhoff, Kremmer, Türeci et al.: "Characterization of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C." in: The Journal of biological chemistry, Vol. 274, Issue 27, pp. 19136-44, 1999 (PubMed).

Charroux, Pellizzoni, Perkinson et al.: "Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems." in: The Journal of cell biology, Vol. 147, Issue 6, pp. 1181-94, 2000 (PubMed).

Meister, Bühler, Laggerbauer et al.: "Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins." in: Human molecular genetics, Vol. 9, Issue 13, pp. 1977-86, 2000 (PubMed).

Ou, Mouillet, Yan et al.: "The DEAD box protein DP103 is a regulator of steroidogenic factor-1." in: Molecular endocrinology (Baltimore, Md.), Vol. 15, Issue 1, pp. 69-79, 2001 (PubMed).