Goat anti-Human Immunoglobulin A, Immunoglobulin G, Immunoglobulin M (IgA, IgG, IgM) (Heavy & Light Chain) Antibody (DyLight 800)

Antigen: Immunoglobulin A, Immunoglobulin G, Immunoglobulin M (IgA, IgG, IgM)

Binding Site: Heavy & Light Chain

Host: Goat

Reactivity: Human

Conjugate: DyLight 800

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN612168

Additional Information

Alternative name: IgG, IgA, IgM

Immunogen: Purified human IgG + IgA + IgM, whole molecule

Format: Lyophilized

Description: Goat anti-human IgG + IgA + IgM (H&L) - Affinity Pure, DyLight 800 Conjugate. Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 800 (Ex = 777 nm, Em = 794 nm). Fluor Protein Ratio: Moles DyLight 800 per Mole Antibody.

Comments:

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

Purity: Affinity purified antibody is > 95% based on SDS-PAGE

Purification: Affinity purified using solid phase goat IgG (H&L)

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide

Storage: Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.

Restrictions: For Research Use only

Publications

Product: Han, Lee, Bibbs et al.: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994 (PubMed).

Brunet, Pouysségur: "Identification of MAP kinase domains by redirecting stress signals into growth factor responses." in: Science (New York, N.Y.), Vol. 272, Issue 5268, pp. 1652-5, 1996 (PubMed).

Morinobu, Gadina, Strober et al.: "STAT4 serine phosphorylation is critical for IL-12-induced IFN-gamma production but not for cell proliferation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 19, pp. 12281-6, 2002 (PubMed).