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Goat anti-Mouse (Murine) IgG (Heavy & Light Chain) Antibody
Heavy & Light Chain
Flow Cytometry (FACS), Immunofluorescence (IF)
|3 references available|
|Quantity||0.5 mg (1 mg/ml (E 1% at 280 nm = 13.0)) (Variants)|
|Price||389.71 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||Purified Mouse IgG, whole molecule|
|Description||Goat anti-Mouse IgG (H&L) - F(ab)'2 fragment, DyLight 650 Conjugate, min x w/bovine, goat, human, rabbit, or rat IgG (highly absorbed against rat IgG). Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 650 (Ex = 652 nm, Em = 672 nm). Fluor Protein Ratio: Moles DyLight 650 per Mole Antibody.|
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
|Application Notes||This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications|
|Concentration||1 mg/ml (E 1% at 280 nm = 13.0)|
|Purity||Affinity purified antibody is > 95% based on SDS-PAGE|
|Purification||Affinity purified using solid phase human IgG (H&L)|
|Buffer||10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide|
|Storage||Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 0.55 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.|
|Research Area||Secondary Antibodies|
|Restrictions||For Research Use only|
Han, Lee, Bibbs et al.: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994 (PubMed).
Brunet, Pouysségur: "Identification of MAP kinase domains by redirecting stress signals into growth factor responses." in: Science (New York, N.Y.), Vol. 272, Issue 5268, pp. 1652-5, 1996 (PubMed).
Winston, Chan, Johnson et al.: "Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4491-7, 1997 (PubMed).