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Goat anti-Mouse (Murine) IgG (Heavy & Light Chain) Antibody (DyLight 800)

Details for Product No. ABIN612290, Supplier: Login to see
Antigen
Epitope
Heavy & Light Chain
4274
1867
1736
964
643
277
203
57
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Mouse (Murine)
2413
2330
2036
1524
1260
609
562
383
328
296
295
291
290
224
162
112
105
76
32
24
21
13
12
8
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
Host
Goat
6021
4118
1527
608
545
262
85
49
43
8
8
4
4
3
1
1
Conjugate
DyLight 800
1870
1685
1511
1095
605
523
517
261
198
180
172
161
156
155
127
79
79
69
43
41
37
32
32
31
23
20
19
19
17
15
14
14
14
14
13
10
10
10
10
10
10
9
9
9
9
8
8
8
8
8
8
8
8
8
8
7
7
6
6
6
6
6
6
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified Mouse IgG, whole molecule
Fragment F(ab')2 fragment
Characteristics Goat anti-Mouse IgG (H&L) - F(ab) '2 fragment, DyLight 800 Conjugate.
Fluorphore: DyLight 800 (Ex = 777 nm, Em = 794 nm).
Fluor Protein Ratio: Moles DyLight 800 per Mole Antibody.
Purification Affinity purified using solid phase human IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C
Product cited in: Chen, Yu, Arawaka et al.: "Nicastrin binds to membrane-tethered Notch." in: Nature cell biology, Vol. 3, Issue 8, pp. 751-4, 2001 (PubMed).

Background publications Yu, Nishimura, Arawaka et al.: "Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing. ..." in: Nature, Vol. 407, Issue 6800, pp. 48-54, 2000 (PubMed).