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Goat anti-Mouse (Murine) IgG (Heavy & Light Chain) Antibody (DyLight 800)

Details for Product No. ABIN612290, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4508
1737
1715
897
817
633
618
279
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
2699
2673
2308
1715
1417
743
613
423
351
337
336
332
312
262
165
149
123
113
42
27
21
21
12
8
7
5
5
5
4
4
4
4
4
4
4
4
4
3
3
2
2
1
1
1
1
Host
Goat
6701
4615
1764
767
653
367
93
52
51
8
8
5
4
3
1
1
Conjugate
DyLight 800
2128
1822
1695
1299
720
633
528
313
307
303
301
283
262
222
216
117
81
79
69
54
49
39
37
25
23
21
19
18
15
15
15
15
14
14
14
14
13
11
10
10
10
10
10
10
9
9
9
9
9
8
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified Mouse IgG, whole molecule
Fragment F(ab')2 fragment
Characteristics Goat anti-Mouse IgG (H&L) - F(ab) '2 fragment, DyLight 800 Conjugate.
Fluorphore: DyLight 800 (Ex = 777 nm, Em = 794 nm).
Fluor Protein Ratio: Moles DyLight 800 per Mole Antibody.
Purification Affinity purified using solid phase human IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C
Product cited in: Chen, Yu, Arawaka, Nishimura, Kawarai, Yu, Tandon, Supala, Song, Rogaeva, Milman, Sato, Yu, Janus, Lee, Song, Zhang, Fraser, St George-Hyslop: "Nicastrin binds to membrane-tethered Notch." in: Nature cell biology, Vol. 3, Issue 8, pp. 751-4, 2001 (PubMed).

Background publications Yu, Nishimura, Arawaka, Levitan, Zhang, Tandon, Song, Rogaeva, Chen, Kawarai, Supala, Levesque, Yu, Yang, Holmes, Milman, Liang, Zhang, Xu, Sato, Rogaev, Smith, Janus, Zhang, Aebersold, Farrer, Sorbi et al.: "Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing. ..." in: Nature, Vol. 407, Issue 6800, pp. 48-54, 2000 (PubMed).