Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (DyLight 350)

Catalog No. ABIN612374

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Conjugate: DyLight 350

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Goat anti-rabbit IgG (H&L) - Affinity Pure, DyLight 350 Conjugate.
Fluorphore: DyLight 350 (Ex = 353 nm, Em = 432 nm).
Fluor Protein Ratio: Moles DyLight 350 per Mole Antibody.

Purification: Affinity purified using solid phase human IgG (H&L)

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified rabbit IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications

Comment:

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C

References

Yuryev, Ono, Goff, Macaluso, Wennogle: "Isoform-specific localization of A-RAF in mitochondria." in: Molecular and cellular biology, Vol. 20, Issue 13, pp. 4870-8, (2000) (PubMed).

Barnier, Papin, Eychène, Lecoq, Calothy: "The mouse B-raf gene encodes multiple protein isoforms with tissue-specific expression." in: The Journal of biological chemistry, Vol. 270, Issue 40, pp. 23381-9, (1995) (PubMed).

Eychène, Dusanter-Fourt, Barnier, Papin, Charon, Gisselbrecht, Calothy: "Expression and activation of B-Raf kinase isoforms in human and murine leukemia cell lines." in: Oncogene, Vol. 10, Issue 6, pp. 1159-65, (1995) (PubMed).

Sithanandam, Kolch, Duh, Rapp: "Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies." in: Oncogene, Vol. 5, Issue 12, pp. 1775-80, (1991) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody