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Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (DyLight 633)

Details for Product No. ABIN612378, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4384
2530
1763
1035
925
824
306
283
35
34
31
8
6
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Rabbit
2656
2627
2285
1633
1385
738
607
422
365
337
333
321
309
259
165
147
124
112
41
27
25
21
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Goat
6521
4534
1762
768
636
365
93
51
50
9
8
5
4
3
1
1
Conjugate
DyLight 633
2119
1814
1663
1306
717
630
522
296
293
284
273
267
216
187
178
117
101
79
69
55
54
40
35
25
23
22
19
19
19
19
18
15
15
15
15
15
15
15
14
13
12
10
10
10
9
9
9
9
9
8
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified rabbit IgG, whole molecule
Characteristics Goat anti-rabbit IgG (H&L) - Affinity Pure, DyLight 633 Conjugate.
Fluorphore: DyLight 633 (Ex = 638 nm, Em = 658 nm).
Fluor Protein Ratio: Moles DyLight 633 per Mole Antibody.
Purification Affinity purified using solid phase human IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1.0 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Product cited in: Rowan, Weigel, OMalley: "Phosphorylation of steroid receptor coactivator-1. Identification of the phosphorylation sites and phosphorylation through the mitogen-activated protein kinase pathway." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4475-83, 2000 (PubMed).

Spencer, Jenster, Burcin, Allis, Zhou, Mizzen, McKenna, Onate, Tsai, Tsai, OMalley: "Steroid receptor coactivator-1 is a histone acetyltransferase." in: Nature, Vol. 389, Issue 6647, pp. 194-8, 1997 (PubMed).

Yao, Ku, Zhou, Scully, Livingston: "The nuclear hormone receptor coactivator SRC-1 is a specific target of p300." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 20, pp. 10626-31, 1996 (PubMed).

Oñate, Tsai, Tsai, OMalley: "Sequence and characterization of a coactivator for the steroid hormone receptor superfamily." in: Science (New York, N.Y.), Vol. 270, Issue 5240, pp. 1354-7, 1995 (PubMed).