Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody

Antigen: IgG

Binding Site: Heavy & Light Chain

Host: Goat

Reactivity: Rabbit

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN612378

Additional Information

Immunogen: Purified rabbit IgG, whole molecule

Format: Lyophilized

Description: Goat anti-rabbit IgG (H&L) - Affinity Pure, DyLight 633 Conjugate. Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 633 (Ex = 638 nm, Em = 658 nm). Fluor Protein Ratio: Moles DyLight 633 per Mole Antibody.

Comments:

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

Purity: Affinity purified antibody is > 95% based on SDS-PAGE

Purification: Affinity purified using solid phase human IgG (H&L)

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide

Storage: Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.

Research Area: Secondary Antibodies

Restrictions: For Research Use only

Publications

Product: Oñate, Tsai, Tsai et al.: "Sequence and characterization of a coactivator for the steroid hormone receptor superfamily." in: Science (New York, N.Y.), Vol. 270, Issue 5240, pp. 1354-7, 1995 (PubMed).

Yao, Ku, Zhou et al.: "The nuclear hormone receptor coactivator SRC-1 is a specific target of p300." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 20, pp. 10626-31, 1996 (PubMed).

Spencer, Jenster, Burcin et al.: "Steroid receptor coactivator-1 is a histone acetyltransferase." in: Nature, Vol. 389, Issue 6647, pp. 194-8, 1997 (PubMed).

Rowan, Weigel, OMalley: "Phosphorylation of steroid receptor coactivator-1. Identification of the phosphorylation sites and phosphorylation through the mitogen-activated protein kinase pathway." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4475-83, 2000 (PubMed).