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Rabbit anti-Guinea Pig IgG (Heavy & Light Chain) Antibody

Antigen

IgG

Binding Site
Alternatives

Heavy & Light Chain

Host
Alternatives

Rabbit

Reactivity
Alternatives

Guinea Pig

Conjugate
Alternatives Un-conjugated
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
3 references available
Catalog no. ABIN612504
Quantity 1 mg  (1.0 mg/ml (E 1% at 280 nm = 13.0))  (Variants)
Price 125.71 $   Plus shipping costs $45.00
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Additional Information

Immunogen Purified guinea pig IgG, whole molecule
Format Lyophilized
Description Rabbit anti-guinea pig IgG (H&L) - Affinity Pure, DyLight 633 Conjugate. Country of Origin: Rabbit serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 633 (Ex = 638 nm, Em = 658 nm). Fluor Protein Ratio: Moles DyLight 633 per Mole Antibody.
Comments

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Application Details

Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications
Concentration 1.0 mg/ml (E 1% at 280 nm = 13.0)
Purity Affinity purified antibody is > 95% based on SDS-PAGE
Purification Affinity purified using solid phase mouse IgG (H&L)
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide
Storage Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.
Research Area Secondary Antibodies
Restrictions For Research Use only

Publications

Product De Simone, De Magistris, Lazzaro et al.: "LFB3, a heterodimer-forming homeoprotein of the LFB1 family, is expressed in specialized epithelia." in: The EMBO journal, Vol. 10, Issue 6, pp. 1435-43, 1991 (PubMed).

Ringeisen, Rey-Campos, Yaniv: "The transactivation potential of variant hepatocyte nuclear factor 1 is modified by alternative splicing." in: The Journal of biological chemistry, Vol. 268, Issue 34, pp. 25706-11, 1994 (PubMed).

Soubt, Marksitzer, Menoud et al.: "Role of tissue-specific transcription factor LFB3 in a cyclic AMP-responsive enhancer of the urokinase-type plasminogen activator gene in LLC-PK1 cells." in: Molecular and cellular biology, Vol. 18, Issue 8, pp. 4698-706, 1998 (PubMed).

Alternatives

Alternatives for antigen "IgG", type "Secondary Antibodies"
Hosts Goat (3758), Rabbit (2824), Donkey (1023), Chicken (371), Mouse (291), Sheep (199), Guinea Pig (29), Rat (20), Pig (14), Duck (6), Horse (5), Cat (Feline) (2), Hamster (1), Human (1)
Reactivities Mouse (Murine) (1409), Human (1361), Rabbit (1142), Rat (Rattus) (949), Goat (707), Guinea Pig (387), Sheep (Ovine) (343), Cow (Bovine) (273), Cat (Feline) (202), Dog (Canine) (197), Horse (Equine) (188), Pig (Porcine) (185), Chicken (172), Hamster (128), Donkey (75), Monkey (70), Golden Syrian Hamster (69), Syrian Hamster (62), Llama (51), Armenian Hamster (27), Ferret (19), Mink (18), Turkey (13), Bird (Avian) (6), Rhesus Monkey (5), Deer (4), Duck (3), Pigeon (3), mouse (Murine) (1)
Conjugates HRP (1238), FITC (1159), Biotin (985), Alkaline Phosphatase (AP) (859), Texas Red (TR) (568), TRITC (376), Rhodamine (369), DyLight 594 (199), DyLight 800 (186), PE (141), DyLight 650 (116), DyLight 633 (115), DyLight 550 (113), DyLight 350 (96), RPE (64), Cy3 (39), Cy5 (39), Gold (37), APC (34), Alexa Fluor 647 (34), Alexa Fluor 350 (33), Alexa Fluor 488 (33), Alexa Fluor 555 (33), Cy5.5 (33), Cy7 (33), PE-Cy3 (33), PE-Cy5 (33), PE-Cy5.5 (33), PE-Cy7 (33), Agarose Beads (27), DyLight 488 (20), SureLight® R-PE (15), DyLight 405 (13), DyLight 680 (13), Atto 425 (10), Atto 488 (10), Atto 532 (10), Atto 550 (10), Atto 594 (10), Atto 647N (10), Atto 655 (10), Colloidal Gold (10nm) (8), Colloidal Gold (5nm) (7), SPRD (7), Beads (6), Peroxidase (POD) (6), Poly-HRP40 (4), beta-Gal (4), Poly-HRP (3), PromoFluor-350 (3), PromoFluor-405 (3), PromoFluor-415 (3), PromoFluor-488 Premium (3), PromoFluor-488LSS (3), PromoFluor-500LSS (3), PromoFluor-505 (3), PromoFluor-510LSS (3), PromoFluor-514LSS (3), PromoFluor-520LSS (3), PromoFluor-532 (3), PromoFluor-546 (3), PromoFluor-555 (3), PromoFluor-590 (3), PromoFluor-610 (3), PromoFluor-633 (3), PromoFluor-647 (3), PromoFluor-670 (3), PromoFluor-680 (3), PromoFluor-700 (3), PromoFluor-750 (3), PromoFluor-770 (3), PromoFluor-780 (3), PromoFluor-840 (3), AMCA (2), Chromeo™ 488 (2), Chromeo™ 546 (2), Colloidal Gold (20nm) (2), SureLight® P3 (2), Chromeo™ 494 (1), Chromeo™ 642 (1), Colloidal Gold (15nm) (1), DTAF (1), FL (1), PE,Cy7 (1), PE,RPE (1), Poly-HRP80 (1)
Epitopes Heavy & Light Chain (3818), Fc Region (766), F(ab')2 Region (649), Fc Region, Chain gamma (78), Heavy Chain (54), Chain gamma (47), H&L (45), Fab Region (39), Chain gamma 1,Chain gamma 2b,Chain gamma 2a,Chain gamma 3 (14), Light Chain (7), Chain gamma,Fc Region (4), N-Term (2), Native Conformation (2), Chain alpha (1), Heavy Chain, Fc Region (1)