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Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 650)

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Antigen
Epitope
Heavy & Light Chain
4392
2530
1765
1035
925
824
306
283
35
34
31
8
6
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Goat
2657
2627
2287
1636
1387
738
607
422
365
337
333
322
310
259
165
147
124
112
41
27
25
21
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Rabbit
6523
4539
1762
768
639
365
93
51
50
9
8
5
4
3
1
1
Conjugate
DyLight 650
2119
1814
1663
1306
717
630
532
296
293
283
274
267
216
187
178
117
101
79
69
55
54
40
35
25
23
22
19
19
19
19
18
15
15
15
15
15
15
15
14
13
12
10
10
10
9
9
9
9
9
8
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified goat IgG, whole molecule
Fragment F(ab')2 fragment
Characteristics Rabbit anti-goat IgG (H&L) - Affinity Pure, DyLight 650 Conjugate, F(ab)2 fragment.
Fluorphore: DyLight 650 (Ex = 652 nm, Em = 672 nm).
Fluor Protein Ratio: Moles DyLight 650 per Mole Antibody.
Purification Affinity purified using solid phase mouse IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Rabbit serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1.0 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Product cited in: Mariner, Anastasiadis, Keilhack, Böhmer, Wang, Reynolds: "Identification of Src phosphorylation sites in the catenin p120ctn." in: The Journal of biological chemistry, Vol. 276, Issue 30, pp. 28006-13, 2001 (PubMed).

Background publications Reynolds, Daniel, McCrea, Wheelock, Wu, Zhang: "Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8333-42, 1994 (PubMed).