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Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody

Details for Product No. ABIN612537, Supplier: Login to see
Antigen
Epitope
Heavy & Light Chain
4274
1867
1736
964
643
277
203
57
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Goat
2414
2330
2036
1524
1259
609
562
383
328
296
295
291
290
224
162
112
105
76
32
24
21
13
12
8
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
Host
Rabbit
6022
4117
1527
608
545
262
85
49
43
8
8
4
4
3
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified goat IgG, whole molecule
Fragment F(ab')2 fragment
Characteristics Rabbit anti-goat IgG (H&L) - Affinity Pure, DyLight 650 Conjugate, F(ab)2 fragment.
Fluorphore: DyLight 650 (Ex = 652 nm, Em = 672 nm).
Fluor Protein Ratio: Moles DyLight 650 per Mole Antibody.
Purification Affinity purified using solid phase mouse IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Rabbit serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1.0 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Product cited in: Mariner, Anastasiadis, Keilhack et al.: "Identification of Src phosphorylation sites in the catenin p120ctn." in: The Journal of biological chemistry, Vol. 276, Issue 30, pp. 28006-13, 2001 (PubMed).

Background publications Reynolds, Daniel, McCrea et al.: "Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8333-42, 1994 (PubMed).