Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody

Antigen: IgG

Binding Site: Heavy & Light Chain

Host: Rabbit

Reactivity: Goat

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN612537

Additional Information

Immunogen: Purified goat IgG, whole molecule

Format: Lyophilized

Description: Rabbit anti-goat IgG (H&L) - Affinity Pure, DyLight 650 Conjugate, F(ab) 2 fragment, min x w/human, mouse, rat IgG. Country of Origin: Rabbit serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 650 (Ex = 652 nm, Em = 672 nm). Fluor Protein Ratio: Moles DyLight 650 per Mole Antibody.

Comments:

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

Purity: Affinity purified antibody is > 95% based on SDS-PAGE

Purification: Affinity purified using solid phase mouse IgG (H&L)

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide

Storage: Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.

Research Area: Secondary Antibodies

Restrictions: For Research Use only

Publications

Product: Reynolds, Daniel, McCrea et al.: "Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8333-42, 1994 (PubMed).

Mariner, Anastasiadis, Keilhack et al.: "Identification of Src phosphorylation sites in the catenin p120ctn." in: The Journal of biological chemistry, Vol. 276, Issue 30, pp. 28006-13, 2001 (PubMed).