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Rabbit anti-Mouse (Murine) IgG (Heavy & Light Chain) Antibody (DyLight 594)

Details for Product No. ABIN612602, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4516
1866
1731
962
643
277
204
57
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
2446
2329
2045
1530
1264
628
561
386
328
310
310
304
293
214
159
112
105
76
32
24
21
13
12
8
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
Host
Rabbit
6065
4197
1507
573
545
298
85
49
43
8
8
4
4
3
1
1
Conjugate
DyLight 594
1916
1711
1552
1135
605
529
523
265
198
181
171
161
156
155
127
79
69
47
43
37
31
23
20
19
19
17
15
14
14
14
14
13
11
10
10
10
10
10
10
9
9
9
9
9
8
8
8
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified mouse IgG, whole molecule
Characteristics Rabbit anti-mouse IgG (H&L) - Affinity Pure, DyLight 594 Conjugate.
Fluorphore: DyLight 594 (Ex = 593 nm, Em = 618 nm).
Fluor Protein Ratio: Moles DyLight 594 per Mole Antibody.
Purification Affinity purified using solid phase mouse IgM (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Rabbit serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1.0 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C
Product cited in: Vazdarjanova, McNaughton, Barnes, Worley, Guzowski: "Experience-dependent coincident expression of the effector immediate-early genes arc and Homer 1a in hippocampal and neocortical neuronal networks." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 23, pp. 10067-71, 2002

Lyford, Yamagata, Kaufmann, Barnes, Sanders, Copeland, Gilbert, Jenkins, Lanahan, Worley: "Arc, a growth factor and activity-regulated gene, encodes a novel cytoskeleton-associated protein that is enriched in neuronal dendrites." in: Neuron, Vol. 14, Issue 2, pp. 433-45, 1995