Sheep anti-Rabbit IgG (H&L) Antibody (DyLight 488)

Antigen: IgG

Epitope: H&L

Host: Sheep

Reactivity: Rabbit

Conjugate: DyLight 488

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN612633

Additional Information

Alternative name: IgG (H&L)

Immunogen: Purified rabbit IgG, whole molecule

Format: Lyophilized

Description: Sheep anti-rabbit IgG (H&L) - Affinity Pure, DyLight 488 Conjugate. Country of Origin: Sheep serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 488 (Ex = 493 nm, Em = 518 nm). Fluor Protein Ratio: Moles DyLight 488 per Mole Antibody.

Comments:

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

Purity: Affinity purified antibody is > 95% based on SDS-PAGE

Purification: Affinity purified using solid phase rabbit lgG (H&L)

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide

Storage: Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.

Research Area: Secondary Antibodies

Restrictions: For Research Use only