Alpha-methylacyl-CoA racemase antibody

Antigen: Alpha-methylacyl-CoA racemase

Clonality: Monoclonal (2F3-C11-B12)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN614201

Additional Information

Gene ID: AMACR

Swiss-Prot: Q9UHK6

Immunogen: Recombinant fragment, corresponding to amino acids 138-319aa of human AMACR (C-terminus).

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2F3-C11-B12

Description: This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)-and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Caution hazardous ingredient: Sodium azide, avoid exposure to skin and eyes, ingestion, and contact with heat, acids, and metals or metal ions. Wash exposed skin with soap and water. Flush eyes with water. Do not discard into plumbing.

Specificity: Mouse monoclonal to AMACR

Application Details

Application Notes: ELISA: 1/5000 – 1/20000. WB: 1/100-1/2000

Concentration: 1. 000mg/ml

Purity: > 90%

Purification: Protein G-Sepharose affinity purified

Buffer: Preservative: 0. 02% Sodium Azide and 50% glycerol, 1% BSA, PBS, PH7. 4.

Storage: Store at -20°C. Avoid repeated freeze / thaw cycles. Ship at 4°-10°

Research Area: Cancer, Signaling, Chromatin and Nuclear Signaling, Cell/Tissue Markers

Restrictions: For Research Use only

Images

AMACR (C-terminus) antibody at 1/1000 dilution, Lane 1: Rat Liver lysate 40ug/Lane, Predicted band size: 42kDa, Observed band size: 42kDa