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Glycogen Synthase Kinase 3 beta (GSK3b) antibody
Human, Rat (Rattus), Mouse (Murine), Monkey
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA
|3 references available|
|Quantity||0.1ml (1. 000mg/ml) (Variants)|
|Price||546.86 $ Plus shipping costs $45.00|
|Immunogen||Purified recombinant fragment of human GSK3B expressed in E. Coli.|
Synonyms: GSK-3 , GSK3-beta.
Glycogen synthase kinase 3 (GSK-3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK-3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK-3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK-3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK-3 are ubiquitously expressed, although particularly high levels of GSK-3beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK-3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines. The activity of GSK is regulated by phosphorylation (Akt: Akt-mediated phosphorylation at Ser21 of GSK-3 and Ser9 of GSK-3 , S6K, RSK, PKA and PKC), dephosphorylation (PP1 and PP2A), and by binding to protein complexes (with beta-catenin, axin, CK1 and the APC complex).
|Specificity||Rabbit polyclonal to HDAC3|
|Synonyms||GSK3B, GPR156, GSK3, GSK-3, C86142, GSK-3beta, 7330414F15Rik, 8430431H08Rik, GSK3beta, gsk3b, fk80d11, GSK-3[b], wu:fb68h05, wu:fk80d11, gsk3, gsk-3, xgsk-3, gsk3beta, MGC131076, gsk3-beta, ATSK42, F12K22.12, F12K22_12, shaggy-like kinase 42, SK42|
|Application Notes||WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400, ELISA: 1/10000|
|Preservative||0.03% sodium azide|
|Storage||Store at 4°C, for long term storage, store at -20°C|
|Restrictions||For Research Use only|
|HDAC3 antibody at 1/2000 dilution + Hela NE lysate 40ug/lane, Predicted band size: 50kDa, Observed band size: 50kDa|
Ikeda, Kishida, Yamamoto et al.: "Axin, a negative regulator of the Wnt signaling pathway, forms a complex with GSK-3beta and beta-catenin and promotes GSK-3beta-dependent phosphorylation of beta-catenin." in: The EMBO journal, Vol. 17, Issue 5, pp. 1371-84, 1998 (PubMed).
Zumbrunn, Kinoshita, Hyman et al.: "Binding of the adenomatous polyposis coli protein to microtubules increases microtubule stability and is regulated by GSK3 beta phosphorylation." in: Current biology : CB, Vol. 11, Issue 1, pp. 44-9, 2001 (PubMed).
Ban, Singh, Krishnan et al.: "GSK-3beta phosphorylation and alteration of beta-catenin in hepatocellular carcinoma." in: Cancer letters, Vol. 199, Issue 2, pp. 201-8, 2003 (PubMed).