p44/42 MAPK (Erk1/2) (AA 750-987) antibody

Antigen: p44/42 MAPK (Erk1/2)

Binding Site: AA 750-987

Clonality: Monoclonal (3F8)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Catalog no.: ABIN614220

Additional Information

Gene ID: 5594

UniProt: P09874

Immunogen: Purified recombinant fragment of human ERBB2 (aa750-987) expressed in E. Coli.

Format: Liquid

Isotype: IgG2b

Clone: 3F8

Description: Synonyms: ERK, p38, p40, p41, ERK2, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, MAPK1.
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.

Specificity: Rabbit polyclonal to PARP1

Molecular Weight: 42kDa

Application Details

Application Notes: WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400, ELISA: 1/10000

Concentration: 1. 000mg/ml

Purity: > 90%

Purification: Ascitic fluid

Preservative: 0.03% sodium azide

Storage: Store at 4°C, for long term storage, store at -20°C

Restrictions: For Research Use only

Images

PARP1 (C-terminus) antibody at 1/5000dilution + 293T whole cell lysate 40ug/Lane, Predicted band size: 113kDa, Observed band size: 113kDa, Additional band size: 200kDa, 70kDa

Publications

Product: Gonzalez, Raden, Rigby et al.: "Heterogeneous expression of four MAP kinase isoforms in human tissues." in: FEBS letters, Vol. 304, Issue 2-3, pp. 170-8, 1992 (PubMed).

Schiemann, Graves, Baumann et al.: "Phosphorylation of the human leukemia inhibitory factor (LIF) receptor by mitogen-activated protein kinase and the regulation of LIF receptor function by heterologous receptor activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 12, pp. 5361-5, 1995 (PubMed).

Lønne, Masoumi, Lennartsson et al.: "Protein kinase Cdelta supports survival of MDA-MB-231 breast cancer cells by suppressing the ERK1/2 pathway." in: The Journal of biological chemistry, Vol. 284, Issue 48, pp. 33456-65, 2009 (PubMed).

Tabakoff, Saba, Printz et al.: "Genetical genomic determinants of alcohol consumption in rats and humans." in: BMC biology, Vol. 7, pp. 70, 2009 (PubMed).