Mouse anti-Human IgE Antibody

Catalog No. ABIN614762

Product Details

Target: IgE

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Precipitation methods and ion exchange chromatography

Purity: > 95 % pure by SDS-PAGE

Immunogen: Purified human IgE

Clone: 4G7

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details

Target: IgE

Abstract: IgE Products

Background: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.Synonyms: Immunoglobulin E human

Molecular Weight: 180 kDa