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Actin antibody (pan)

Details for Product anti-Actin Antibody No. ABIN614804, Supplier: Log in to see
Antigen
  • 42A
  • 143060_f_at
  • A
  • Act
  • Act(88F)
  • Act42
  • act42A
  • act 42A
  • Act42a
  • Act57
  • act57A
  • Act57A
  • act 57A
  • Act57b
  • act57B
  • act57b
  • act 79B
  • act79B
  • Act87e
  • act 87E
  • act87E
  • Act88-F
  • Act88F
  • act88F
  • act 88F
  • Act88f
  • Actin
  • actin
  • Actin/BAP47
  • AFFX-Dros-ACTIN_M_r_at
  • anon-EST:CL2c12
  • BAP47
  • BEST:RE38067
  • C
  • CG5178
  • CG7478
  • CG10067
  • CG12051
  • CG18290
  • CG30294
  • cytoplasmic
  • D
  • Dmel\\CG5178
  • Dmel\\CG7478
  • Dmel\\CG10067
  • Dmel\\CG12051
  • Dmel\\CG18290
  • Dsim\\GD19025
  • dsim_GLEANR_2829
  • E
  • GD19025
  • Ifm(3)7
  • M
  • mus79B
  • mus87E
  • rsd
  • T10
Epitope
pan
38
29
20
14
13
8
5
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Chicken, Dog (Canine), Human, Mouse (Murine), Primate, Rabbit, Rat (Rattus)
278
202
136
122
73
49
32
28
26
21
19
18
18
16
16
16
15
15
5
5
5
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
227
99
5
2
Clonality (Clone)
Monoclonal ()
Conjugate
This Actin antibody is un-conjugated
13
10
9
7
7
7
6
6
6
6
6
6
6
6
6
6
6
3
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
255
144
120
79
76
76
69
61
55
12
5
4
3
1
1
1
Supplier
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Available images

Immunogen SDS extracted protein fraction of Human myocardium.
Clone HHF35
Isotype IgG1
Cross-Reactivity (Details) Species reactivity (tested):Human, Non-Human Primates, Mouse, Rat, Canine (Dog), Feline (Cat), Rabbit, Chicken.
Purification Protein-A Affinity Chromatography
Background Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.
Research Area Cancer, Apoptosis/Necrosis, Cytoskeleton, Microfilaments, Extracellular Matrix
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH ~7.4, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Actin antibody (pan) (ABIN614804) anti-Actin (pan) antibody
Background publications Matsuo, Susumu, Tsutsumi, Azuma, Obata, Hayashi: "Glomus tumor of the omentum: a case report." in: Journal of surgical oncology, Vol. 96, Issue 7, pp. 633-6, 2007 (PubMed).

Matsuyama, Hisaoka, Hashimoto: "Angioleiomyoma: a clinicopathologic and immunohistochemical reappraisal with special reference to the correlation with myopericytoma." in: Human pathology, Vol. 38, Issue 4, pp. 645-51, 2007 (PubMed).

Ijzer, Roskams, Molenbeek, Ultee, Penning, Rothuizen, van den Ingh: "Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver." in: Comparative hepatology, Vol. 5, pp. 7, 2006 (PubMed).

Handharyani, Ochiai, Iwata, Umemura: "Immunohistochemical and ultrastructural study of ito cells (fat-storing cells) in response to extrahepatic bile duct ligation in broiler chickens." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 63, Issue 5, pp. 547-52, 2001 (PubMed).

de Sousa, Schwarzschild, de Araújo, de Araújo: "Basal cell adenocarcinoma of the palate with squamous metaplasia." in: Journal of clinical pathology, Vol. 53, Issue 2, pp. 153-6, 2000 (PubMed).

Schmidt, Cone, Haas, Gown: "Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins." in: The American journal of pathology, Vol. 131, Issue 1, pp. 19-28, 1988 (PubMed).

Miettinen: "Antibody specific to muscle actins in the diagnosis and classification of soft tissue tumors." in: The American journal of pathology, Vol. 130, Issue 1, pp. 205-15, 1988 (PubMed).

Tsukada, Tippens, Gordon, Ross, Gown: "HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization." in: The American journal of pathology, Vol. 126, Issue 1, pp. 51-60, 1987 (PubMed).

Tsukada, McNutt, Ross, Gown: "HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues." in: The American journal of pathology, Vol. 127, Issue 2, pp. 389-402, 1987 (PubMed).