NEFH antibody

Catalog No. ABIN614809

Product Details anti-NEFH Antibody

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), ELISA (Capture)

Cross-Reactivity (Details): Species reactivity (tested):Human, Porcine, Rat.

Purification: Precipitation methods and size-exclusion chromatography

Purity: > 95 % pure by SDS-PAGE

Immunogen: Pig brain neurofilament protein-enriched fraction after depolymerization of microtubules

Clone: NF-05

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE.

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: Neurofilament H (200 kD) (NEFH Products)

Synonyms: NF-H antibody, NF200 antibody, Nfh antibody, mKIAA0845 antibody, NFH antibody, NEFH antibody, neurofilament heavy polypeptide antibody, neurofilament, heavy polypeptide antibody, neurofilament heavy antibody, MCYG_06231 antibody, Nefh antibody, NEFH antibody

Background: Neurofilaments (NFs) are a type of intermediate filaments (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights - neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.Synonyms: (Neuronal Marker), 200 kDa neurofilament protein, KIAA0845, NEFH, NF-H, NFH, Neurofilament triplet H protein, heavy polypeptide

Gene ID: 9823

UniProt: P12037