UBE2F antibody (N-Term)

Catalog No. ABIN615974

Product Details anti-UBE2F Antibody

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: SKLKRDDGLK GSRT-C

Specificity: Recogfnizes NEDD8 conjugating enzyme (N Terminus).

Cross-Reactivity (Details): Species reactivity (expected):Human, Mouse, Canine (Dog), Pig, Cow.
Species reactivity (tested):Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_542409.1. Genename: UBE2F

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: NCE2 (UBE2F Products)

Synonyms: NCE2 antibody, 2510010F15Rik antibody, AI851109 antibody, RGD1307608 antibody, zgc:77005 antibody, ubiquitin conjugating enzyme E2 F (putative) antibody, NEDD8-conjugating enzyme UBE2F antibody, ubiquitin-conjugating enzyme E2F (putative) antibody, ubiquitin conjugating enzyme E2F (putative) S homeolog antibody, UBE2F antibody, Tsp_01616 antibody, ube2f antibody, ube2f.S antibody, Ube2f antibody

Background: Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43 % sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.Synonyms: NEDD8-conjugating enzyme 2, UBE2F, Ubiquitin-conjugating enzyme E2 F

Gene ID: 140739

NCBI Accession: NP_542409

UniProt: Q969M7