Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)
Specificity
This antibody detects recombinant human soluble CEACAM-1. The antibody shows cross-reactivity with several other CEACAM familiy members. There is no cross-reactivity with human CEACAM-8.
Cross-Reactivity (Details)
Species reactivity (tested):Human
Purification
Protein A affinity chromatography
Immunogen
Highly pure recombinant human soluble CEACAM-1 (D1-4)
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS pH 7.4 w/o preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
The lyophilized antibody can be stored at room temperature for up to two weeks, or desiccated at -20 °C for longer. Following reconstitution it is stable at 2 - 8 °C for up to six moths or (in aliquots) at -20 °C for longer.
Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM-1), also known as CD66a and BGP-I, is a 115 kDa type I transmembrane glycoprotein that belongs to the membrane-bound CEA subfamily of the CEA superfamily. On the surface of cells, CEACAM-1 forms non-covalent homo- and heterodimers. The extracellular region contains three C2-type Ig-like domains and one N-terminal V-type Ig-like domain. Multiple splice of CEACAM-1 variants involving regions C-terminal to the second C2-type domain (aa 320 and beyond) exist.Synonyms: BGP, BGP1, Biliary glycoprotein 1, Carcinoembryonic antigen-related cell adhesion molecule 1