ESAM antibody (AA 30-248)

Catalog No. ABIN616014

Product Details anti-ESAM Antibody

Target: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Binding Specificity: AA 30-248

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESAM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody will detect recombinant human soluble ESAM in Western blot and native ESAM in Immunoflourescence.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A affinity chromatography

Immunogen: Highly pure (>95%) recombinant human ESAM (Ile30-Ala248) derived from insect cells.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS pH 7.4 w/o preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for up to 1 month. Following reconstitution antibody can be stored at 2-8 °C for up to two weeks or (in aliquots) at -20 °C for longer.

Target Details for ESAM

Target: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Alternative Name: ESAM (ESAM Products)

Synonyms: ESAM antibody, sc:d0254 antibody, W117m antibody, 2310008D05Rik antibody, Esam1 antibody, endothelial cell adhesion molecule antibody, endothelial cell adhesion molecule a antibody, endothelial cell-specific adhesion molecule antibody, ESAM antibody, esama antibody, Esam antibody

Background: Endothelial cellselective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules. Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in hemophilic adhesion. In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI1. The extracellular region of human ESAM shows 90 % , 74 %, 69 % and 67 % aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cell to cell junctions. Whether ESAM is restricted to a particular junctional type is not clear. ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF2. Soluble ESAM is fused to a C-terminal His-tag (6x His).Synonyms: Endothelial cell-selective adhesion molecule

Gene ID: 90952

NCBI Accession: NP_620411

UniProt: Q96AP7