GM-CSF antibody (AA 18-141)

Catalog No. ABIN616015

This Rabbit Polyclonal antibody specifically detects GM-CSF in WB. It exhibits reactivity toward Mouse.

Quick Overview for GM-CSF antibody (AA 18-141) (ABIN616015)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GM-CSF Antibody

Binding Specificity: AA 18-141

Specificity: This antibody detects GM-CSF.

Cross-Reactivity (Details): Species reactivity (tested):Mouse

Purification: Protein-A purified

Immunogen: Highly pure (>95%) recombinant mouse GM-CSF (Ala18-Lys141) derived from E. coli

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for up to 1 month. Following reconstitution antibody can be stored at 2-8 °C for up to two weeks or (in aliquots) at -20 °C for longer.

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Background: GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils. GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines. The 22 kDa glycosylated GM-CSF, similar to IL3 and IL5, is a cytokine with a core of four bundled α-helices. Mature mouse GM-CSF shares 49-54 % amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69 % with rat GMCSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF Rα/CD116 and the signal transducing common β chain (CD131) which is also a component of the high affinity receptors for IL3 and IL5. In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF Rα. The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

Gene ID: 12981

NCBI Accession: NP_034099

UniProt: P01587

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin