HTR2A antibody (AA 22-41)

Catalog No. ABIN617912

Product Details anti-HTR2A Antibody

Target: HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))

Binding Specificity: AA 22-41

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTR2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)

Cross-Reactivity: Mouse (Murine), Rabbit, Rat (Rattus)

Characteristics: The 5-HT 2A Receptor Antibody was raised against a multiple antigenic peptide of an N-terminal synthetic sequence corresponding to amino acids 22-41 of rat 5HT2A receptor. The antibody is provided as 100 μLof affinity purified serum in PBS (0.02 M sodium phosphate with 0.15 M sodium chloride, pH 7.5) with 1 % BSA (bovine serum albumin), and 0.02 % sodium azide. The antiserum demonstrates strongly positive labeling of rat cortex, amygdala and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/300 - 1/500 in PBS/0.03 % Triton X-100 - Bn/Av-HRP Technique. The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution will approximately double the dilution factor as recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT2A receptor (22-41). Immunolabeling of Western blot revealed a single band of approximately 53kD.

Purification: Affinity Purified

Immunogen: Rat 5HT2A receptor (22-41) MAP

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Contains ≤ 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.

References for anti-HTR2A antibody (ABIN617912)

Cao, Ni, Wang, Tu, Gu, Si, Wu, Andersson: "Chronic spinal cord injury causes upregulation of serotonin (5-HT)2A and 5-HT2C receptors in lumbosacral cord motoneurons." in: BJU international, Vol. 121, pp. 145-154, (2017) (PubMed).

Karaki, Becamel, Murat, Mannoury la Cour, Millan, Prézeau, Bockaert, Marin, Vandermoere: "Quantitative phosphoproteomics unravels biased phosphorylation of serotonin 2A receptor at Ser280 by hallucinogenic versus nonhallucinogenic agonists." in: Molecular & cellular proteomics : MCP, Vol. 13, Issue 5, pp. 1273-85, (2015) (PubMed).

Rasul, Johansson, Lonne-Rahm, Nordlind, Theodorsson, El-Nour: "Chronic mild stress modulates 5-HT1A and 5-HT2A receptor expression in the cerebellar cortex of NC/Nga atopic-like mice." in: Archives of dermatological research, Vol. 305, Issue 5, pp. 407-13, (2013) (PubMed).

Sławińska, Miazga, Cabaj, Leszczyńska, Majczyński, Nagy, Jordan: "Grafting of fetal brainstem 5-HT neurons into the sublesional spinal cord of paraplegic rats restores coordinated hindlimb locomotion." in: Experimental neurology, Vol. 247, pp. 572-81, (2013) (PubMed).

Volgin, Stettner, Kubin: "Circadian dependence of receptors that mediate wake-related excitatory drive to hypoglossal motoneurons." in: Respiratory physiology & neurobiology, Vol. 188, Issue 3, pp. 301-7, (2013) (PubMed).

Stettner, Kubin, Volgin: "Loss of motoneurons in the ventral compartment of the rat hypoglossal nucleus following early postnatal exposure to alcohol." in: Journal of chemical neuroanatomy, Vol. 52, pp. 87-94, (2013) (PubMed).

Maekawa, Komori, Kajikuri, Itoh: "Characteristics of the actions by which 5-hydroxytryptamine affects electrical and mechanical activities in rabbit jugular vein graft." in: British journal of pharmacology, Vol. 166, Issue 4, pp. 1419-32, (2012) (PubMed).

Loyd, Sun, Locke, Salas, Hargreaves: "Sex differences in serotonin enhancement of capsaicin-evoked calcitonin gene-related peptide release from human dental pulp." in: Pain, Vol. 153, Issue 10, pp. 2061-7, (2012) (PubMed).

Dumitrascu, Kulcke, Königshoff, Kouri, Yang, Morrell, Ghofrani, Weissmann, Reiter, Seeger, Grimminger, Eickelberg, Schermuly, Pullamsetti: "Terguride ameliorates monocrotaline-induced pulmonary hypertension in rats." in: The European respiratory journal, Vol. 37, Issue 5, pp. 1104-18, (2011) (PubMed).

Kong, Wienecke, Chen, Hultborn, Zhang: "The time course of serotonin 2A receptor expression after spinal transection of rats: an immunohistochemical study." in: Neuroscience, Vol. 177, pp. 114-26, (2011) (PubMed).

Coulon, Kanyshkova, Broicher, Munsch, Wettschureck, Seidenbecher, Meuth, Offermanns, Pape, Budde: "Activity Modes in Thalamocortical Relay Neurons are Modulated by G(q)/G(11) Family G-proteins - Serotonergic and Glutamatergic Signaling." in: Frontiers in cellular neuroscience, Vol. 4, pp. 132, (2011) (PubMed).

Itoh, Kajikuri: "Characteristics of the actions by which 5-HT affects electrical and mechanical activities in rabbit jugular vein." in: British journal of pharmacology, Vol. 164, Issue 3, pp. 979-91, (2011) (PubMed).

Pearlstein, Bras, Deneris, Vinay: "Contribution of 5-HT to locomotion - the paradox of Pet-1(-/-) mice." in: The European journal of neuroscience, Vol. 33, Issue 10, pp. 1812-22, (2011) (PubMed).

Rasul, El-Nour, Blakely, Lonne-Rahm, Forsberg, Johansson, Theodorsson, Nordlind: "Effect of chronic mild stress on serotonergic markers in the skin and brain of the NC/Nga atopic-like mouse strain." in: Archives of dermatological research, Vol. 303, Issue 9, pp. 625-33, (2011) (PubMed).

Matsumoto, Kobayashi, Ishida, Taguchi, Kamata: "Enhancement of mesenteric artery contraction to 5-HT depends on Rho kinase and Src kinase pathways in the ob/ob mouse model of type 2 diabetes." in: British journal of pharmacology, Vol. 160, Issue 5, pp. 1092-104, (2010) (PubMed).

Weber, Andrade: "Htr2a Gene and 5-HT(2A) Receptor Expression in the Cerebral Cortex Studied Using Genetically Modified Mice." in: Frontiers in neuroscience, Vol. 4, (2010) (PubMed).

Kong, Wienecke, Hultborn, Zhang: "Robust upregulation of serotonin 2A receptors after chronic spinal transection of rats: an immunohistochemical study." in: Brain research, Vol. 1320, pp. 60-8, (2010) (PubMed).

Van Steenwinckel, Noghero, Thibault, Brisorgueil, Fischer, Conrath: "The 5-HT2A receptor is mainly expressed in nociceptive sensory neurons in rat lumbar dorsal root ganglia." in: Neuroscience, Vol. 161, Issue 3, pp. 838-46, (2009) (PubMed).

Kodama, Komori, Kajikuri, Itoh: "Chronic treatment of hydroxytryptamine type 2a receptor antagonist sarpogrelate hydrochloride modulates the vasoreactivity of serotonin in experimental rabbit vein grafts." in: Journal of vascular surgery, Vol. 50, Issue 3, pp. 617-25, (2009) (PubMed).

Van Steenwinckel, Brisorgueil, Fischer, Vergé, Gingrich, Bourgoin, Hamon, Bernard, Conrath: "Role of spinal serotonin 5-HT2A receptor in 2',3'-dideoxycytidine-induced neuropathic pain in the rat and the mouse." in: Pain, Vol. 137, Issue 1, pp. 66-80, (2008) (PubMed).

Target Details for HTR2A

Target: HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))

Alternative Name: Serotonin Receptor 2A (HTR2A) (HTR2A Products)

Synonyms: 5-HT2A antibody, HTR2 antibody, 5-HT-2 antibody, 5-HT-2A antibody, 5-HTR2A antibody, LOC100009461 antibody, E030013E04 antibody, Htr-2 antibody, Htr2 antibody, 5Ht-2 antibody, 5HT2A antibody, 5HTR2A antibody, HTR2A antibody, Htr2a antibody, 5HT2 antibody, 5-hydroxytryptamine receptor 2A antibody, 5-hydroxytryptamine (serotonin) receptor 2A antibody, HTR2A antibody, Htr2a antibody

Background: Other Names:
5-hydroxytryptamine receptor 2A,5Ht-2,5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled

Gene ID: 29595

Pathways: JAK-STAT Signaling, Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process