HTR7 antibody (AA 8-23)

Catalog No. ABIN617922

Product Details anti-HTR7 Antibody

Target: HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))

Binding Specificity: AA 8-23

Reactivity: Human, Rat, Mouse, Pig, Cat, Grasshopper, Rabbit, Shrimp

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTR7 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Characteristics: The 5-HT7 Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 8-23 of the rat 5-HT7 receptor coupled to carrier protein with glutaraldehyde. The 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS - biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended. The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.

Purification: Affinity Purified

Immunogen: Rat 5-HT7 receptor (8-23)

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Contains ≤ 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.

References for anti-HTR7 antibody (ABIN617922)

Korlatowicz, Pabian, Solich, Kolasa, Latocha, Dziedzicka-Wasylewska, Faron-Górecka: "Habenula as a Possible Target for Treatment-Resistant Depression Phenotype in Wistar Kyoto Rats." in: Molecular neurobiology, Vol. 60, Issue 2, pp. 643-654, (2023) (PubMed).

Maekawa, Komori, Kajikuri, Itoh: "Characteristics of the actions by which 5-hydroxytryptamine affects electrical and mechanical activities in rabbit jugular vein graft." in: British journal of pharmacology, Vol. 166, Issue 4, pp. 1419-32, (2012) (PubMed).

Khalmuratova, Hah, Ahn, Jeon, Kim, Balaban: "Immunohistochemical and biomolecular identification of 5-HT₇ receptor in rat vestibular nuclei." in: Journal of vestibular research : equilibrium & orientation, Vol. 20, Issue 6, pp. 401-6, (2011) (PubMed).

Itoh, Kajikuri: "Characteristics of the actions by which 5-HT affects electrical and mechanical activities in rabbit jugular vein." in: British journal of pharmacology, Vol. 164, Issue 3, pp. 979-91, (2011) (PubMed).

Brenchat, Nadal, Romero, Ovalle, Muro, Sánchez-Arroyos, Portillo-Salido, Pujol, Montero, Codony, Burgueño, Zamanillo, Hamon, Maldonado, Vela: "Pharmacological activation of 5-HT7 receptors reduces nerve injury-induced mechanical and thermal hypersensitivity." in: Pain, Vol. 149, Issue 3, pp. 483-94, (2010) (PubMed).

Noga, Johnson, Riesgo, Pinzon: "Locomotor-activated neurons of the cat. I. Serotonergic innervation and co-localization of 5-HT7, 5-HT2A, and 5-HT1A receptors in the thoraco-lumbar spinal cord." in: Journal of neurophysiology, Vol. 102, Issue 3, pp. 1560-76, (2009) (PubMed).

Iwasato, Inan, Kanki, Erzurumlu, Itohara, Crair: "Cortical adenylyl cyclase 1 is required for thalamocortical synapse maturation and aspects of layer IV barrel development." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 23, pp. 5931-43, (2008) (PubMed).

Parga, Rodriguez-Pallares, Muñoz, Guerra, Labandeira-Garcia: "Serotonin decreases generation of dopaminergic neurons from mesencephalic precursors via serotonin type 7 and type 4 receptors." in: Developmental neurobiology, Vol. 67, Issue 1, pp. 10-22, (2007) (PubMed).

Doly, Fischer, Brisorgueil, Vergé, Conrath: "Pre- and postsynaptic localization of the 5-HT7 receptor in rat dorsal spinal cord: immunocytochemical evidence." in: The Journal of comparative neurology, Vol. 490, Issue 3, pp. 256-69, (2005) (PubMed).

Rocha-González, Meneses, Carlton, Granados-Soto: "Pronociceptive role of peripheral and spinal 5-HT7 receptors in the formalin test." in: Pain, Vol. 117, Issue 1-2, pp. 182-92, (2005) (PubMed).

Muneoka, Takigawa: "5-Hydroxytryptamine7 (5-HT7) receptor immunoreactivity-positive 'stigmoid body'-like structure in developing rat brains." in: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, Vol. 21, Issue 3, pp. 133-43, (2003) (PubMed).

Turner, Alvarez, Candia, Bernstein: "Characterization of serotonergic receptors in rabbit, porcine and human conjunctivae." in: Current eye research, Vol. 27, Issue 4, pp. 205-15, (2003) (PubMed).

Belenky, Pickard: "Subcellular distribution of 5-HT(1B) and 5-HT(7) receptors in the mouse suprachiasmatic nucleus." in: The Journal of comparative neurology, Vol. 432, Issue 3, pp. 371-88, (2001) (PubMed).

Contesse, Lenglet, Grumolato, Anouar, Lihrmann, Lefebvre, Delarue, Vaudry: "Pharmacological and molecular characterization of 5-hydroxytryptamine(7) receptors in the rat adrenal gland." in: Molecular pharmacology, Vol. 56, Issue 3, pp. 552-61, (1999) (PubMed).

Condron, Zinn: "Regulated neurite tension as a mechanism for determination of neuronal arbor geometries in vivo." in: Current biology : CB, Vol. 7, Issue 10, pp. 813-6, (1998) (PubMed).

Target Details for HTR7

Target: HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))

Alternative Name: Serotonin Receptor 7 (HTR7) (HTR7 Products)

Background: Other Names:
5-hydroxytryptamine receptor 7,5-HT-X,GPRFO,5Ht7,5-hydroxytryptamine (serotonin) receptor 7

Gene ID: 65032

Pathways: JAK-STAT Signaling