TRIM71 antibody (AA 390-402)

Catalog No. ABIN625883

Product Details anti-TRIM71 Antibody

Target: TRIM71 (Tripartite Motif Containing 71, E3 Ubiquitin Protein Ligase (TRIM71))

Binding Specificity: AA 390-402

Reactivity: Human, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Monkey, Bat, Chicken

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM71 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TRIM71.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Lizard, Pufferfish, Zebrafish (100%) Mouse, Xenopus, Stickleback (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RQVKAKSLYLQVE from an internal region of human TRIM71 (NP_001034200.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Lizard, Pufferfish, Zebrafish (100%), Mouse, Xenopus, Stickleback (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:8000), WB (0.5 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 100  kDa band observed in lysates of the cell lines HEK293 and NIH3T3 (calculated MW of 93.4  kDa according to NP_001034200.1). The observed molecular weight corresponds to findings with EB09902, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.5-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TRIM71

Target: TRIM71 (Tripartite Motif Containing 71, E3 Ubiquitin Protein Ligase (TRIM71))

Alternative Name: LIN41 / TRIM71 (TRIM71 Products)

Synonyms: LIN-41 antibody, LIN41 antibody, RGD1566388 antibody, 2610206G21Rik antibody, AL022943 antibody, Gm1127 antibody, Lin41 antibody, Ripply2 antibody, lin-41 antibody, mLin41 antibody, mlin-41 antibody, tripartite motif containing 71 antibody, tripartite motif-containing 71 antibody, TRIM71 antibody, Trim71 antibody

Background: Name/Gene ID: TRIM71

Synonyms: TRIM71, Abnormal cell LINeage LIN-41, Homolog of C. elegans Lin-41, Protein lin-41 homolog, LIN41, Tripartite motif containing 71, Tripartite motif-containing 71, LIN-41

Gene ID: 131405

NCBI Accession: NP_001034200

UniProt: Q2Q1W2

Pathways: Tube Formation