NSMCE2 antibody

Catalog No. ABIN631769

Product Details anti-NSMCE2 Antibody

Target: NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSMCE2 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Affinity purified

Immunogen: NSMCE2 antibody was raised using a synthetic peptide corresponding to a region with amino acids KFLALQSKNSDADFQNNEKFVQFKQQLKELKKQCGLQADREADGTEGVDE

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

NSMCE2 Blocking Peptide, catalog no. 33R-4372, is also available for use as a blocking control in assays to test for specificity of this NSMCE2 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of NSMCE2 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for NSMCE2

Target: NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))

Alternative Name: NSMCE2 (NSMCE2 Products)

Synonyms: 1110014D18Rik antibody, AI661537 antibody, RGD1305156 antibody, C8orf36 antibody, MMS21 antibody, NSE2 antibody, ZMIZ7 antibody, Non-SMC element 2 antibody, NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase antibody, NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase L homeolog antibody, Smc5-6 complex non-SMC subunit 2 antibody, Nsmce2 antibody, NSMCE2 antibody, nsmce2.L antibody, nse2 antibody

Background: NSMCE2 is the E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. NSMCE2 acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.

Molecular Weight: 28 kDa (MW of target protein)