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LAMP1 antibody (Lysosomal-Associated Membrane Protein 1) (PE)

Details for Product anti-LAMP1 Antibody No. ABIN638446, Supplier: Log in to see
Antigen
  • AI196048
  • CD107a
  • Lamp-1
  • LAMPA
  • LEP100
  • LGP120
Reactivity
Human, Non-Human Primate, Mouse (Murine)
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197
140
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38
13
2
2
Host
Mouse
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66
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This LAMP1 antibody is conjugated to PE
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36
28
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13
12
9
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2
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2
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2
1
1
1
1
1
1
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1
1
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1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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14
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12
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1
Supplier
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Immunogen Human PBMC
Clone H4A3
Isotype IgG1
Specificity The mouse monoclonal antibody H4A3 recognizes CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name LAMP1 (LAMP1 Antibody Abstract)
Background CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2 % of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
Gene ID 3916
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 4 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (0.4 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 0.02 % sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-LAMP1 antibody (Lysosomal-Associated Membrane Protein 1)  (PE) (ABIN638446) Surface staining of human peripheral blood cells with anti-CD107a (H4A3) PE.
Product cited in: Majer, Vlaskova, Krol, Kalina, Kubanek, Stolnaya, Dvorakova, Elleder, Sikora: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." in: Gene, Vol. 498, Issue 2, pp. 183-95, 2012 (PubMed).

Mao, Tu, Liu, Qin, Zheng, Chan, Lam, Peiris, Lau: "Inhibition of human natural killer cell activity by influenza virions and hemagglutinin." in: Journal of virology, Vol. 84, Issue 9, pp. 4148-57, 2010 (PubMed).

Yu, Gallegos, Marches, Zurawski, Ramilo, García-Sastre, Banchereau, Palucka: "Broad influenza-specific CD8+ T-cell responses in humanized mice vaccinated with influenza virus vaccines." in: Blood, Vol. 112, Issue 9, pp. 3671-8, 2008 (PubMed).

Carlsten, Björkström, Norell, Bryceson, van Hall, Baumann, Hanson, Schedvins, Kiessling, Ljunggren, Malmberg: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." in: Cancer research, Vol. 67, Issue 3, pp. 1317-25, 2007 (PubMed).

Tomescu, Chehimi, Maino, Montaner: "NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 4, pp. 2097-104, 2007 (PubMed).

Marcenaro, Gallo, Martini, Santoro, Griffiths, Aricó, Moretta, Pende: "Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease." in: Blood, Vol. 108, Issue 7, pp. 2316-23, 2006 (PubMed).

Deetz, Hebbeler, Propp, Cairo, Tikhonov, Pauza: "Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys." in: Infection and immunity, Vol. 74, Issue 8, pp. 4505-11, 2006 (PubMed).

Furuta, Ikeda, Nakayama, Nakamura, Tanaka, Hamasaki, Himeno, Hamilton, August: "Expression of lysosome-associated membrane proteins in human colorectal neoplasms and inflammatory diseases." in: The American journal of pathology, Vol. 159, Issue 2, pp. 449-55, 2001 (PubMed).

Mane, Marzella, Bainton, Holt, Cha, Hildreth, August: "Purification and characterization of human lysosomal membrane glycoproteins." in: Archives of biochemistry and biophysics, Vol. 268, Issue 1, pp. 360-78, 1989 (PubMed).