14-3-3 b/a (N-Term) antibody

Antigen: 14-3-3 b/a

Binding Site: N-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649226

Additional Information

Gene ID: 7529

UniProt: P31946

Description: Other names: YWHAB, 14-3-3 protein beta/alpha, N-terminally processed, Protein 1054

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near N-terminus of human 14-3-3 beta/alpha was used as immunogen. Predicted to cross-react with sheep and bovine, based on sequence homology.

Molecular Weight: 30 kDA

Comments:

Background: The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3)

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 FC: ~~ 1:50 IHC: ~~ 1:250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: 14-3-3 b/a Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Muslin, Tanner, Allen et al.: "Interaction of 14-3-3 with signaling proteins is mediated by the recognition of phosphoserine." in: Cell, Vol. 84, Issue 6, pp. 889-97, 1996 (PubMed).

Zha, Harada, Yang et al.: "Serine phosphorylation of death agonist BAD in response to survival factor results in binding to 14-3-3 not BCL-X(L)" in: Cell, Vol. 87, Issue 4, pp. 619-28, 1997 (PubMed).

Fu, Subramanian, Masters: "14-3-3 proteins: structure, function, and regulation." in: Annual review of pharmacology and toxicology, Vol. 40, pp. 617-47, 2000 (PubMed).