|+1 404 474 4654|
|+1 888 205 9894 (TF)|
tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, epsilon Polypeptide (YWHAE) antibody
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)
|4 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Gene ID||93659, 94027, 1082, 94115|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues in human 14-3-3E was used as an immunogen.|
|Molecular Weight||30 kDA|
Background: The 14-3-3 epsilon protein (14-3-3E) is one of seven isoforms in the 14-3-3 protein family, a family which consists of acidic 30 kDa proteins expressed at high levels in neurons of the central nervous system (1). It is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3E binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (2). It also interacts with both, the insulin-like growth factor I receptor (IGFIR) and the insulin receptor substrate I (IRS-1), suggesting its role in the regulation of insulin sensitivity (3). 14-3-3E has also been implicated in the pathogenesis of small cell lung cancer (4).
|Synonyms||MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465, AU019196, 14-3-3e, 14-3-3, 14-3-3-e, CT24092, D14-3-3e, D14-3-3epsilon, EK3-5, PAR5, Par-5, SR3-9, Su(Raf)3B, anon-WO0172774.141, anon-WO02059370.52, l(3)j2B10, DmelCG31196, CG31196, LOC443300, YWHAE, mds, mdcr, kcip-1, MGC89915, 14-3-3epsilon, LOC581376, Ywhae, ACYPI002517, ywhae, zgc:55377, zgc:77062, wu:fb48f06, wu:fi22e10, MGC53170|
|Application Notes||The suggested dilution is: WB: ~~ 1:20000~100000 IP: ~~ 1:30 FC: ~~ 1:100 ICC: ~~ 1:100~250|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||14-3-3E Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Craparo, Freund, Gustafson: "14-3-3 (epsilon) interacts with the insulin-like growth factor I receptor and insulin receptor substrate I in a phosphoserine-dependent manner." in: The Journal of biological chemistry, Vol. 272, Issue 17, pp. 11663-9, 1997 (PubMed).
Satoh, Yamamura, Arima: "The 14-3-3 protein epsilon isoform expressed in reactive astrocytes in demyelinating lesions of multiple sclerosis binds to vimentin and glial fibrillary acidic protein in cultured human astrocytes." in: The American journal of pathology, Vol. 165, Issue 2, pp. 577-92, 2004 (PubMed).
Pruitt, Tatusova, Maglott: "NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins." in: Nucleic acids research, Vol. 35, Issue Database issue, pp. D61-5, 2007 (PubMed).
"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).
|Hosts||Rabbit (51), Mouse (7), Sheep (1)|
|Reactivities||Human (48), Mouse (Murine) (27), Rat (Rattus) (26), Sheep (Ovine) (19), Cow (Bovine) (18), Chicken (15), Dog (Canine) (2), Cat (Feline) (1), Rabbit (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (43), ELISA (31), Immunofluorescence (IF) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Immunohistochemistry (IHC) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (10), pThr232 (6), N-Term (5), AA 1-255 (3), AA 208-244 (2), pSer58 (2), Internal Region (1), pSer185 (1), pSer53 (1)|