Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) antibody

Antigen: Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9)

Synonyms: CD137L, 4-1BB-L, Ly63l, 4-1BBL, Cd137l, AI848817, TNFSF9

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649231

Additional Information

Alternative name: 4-1BBL

Gene ID: 8744

UniProt: P41273

Description: Other names: TNFSF9, Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the N-terminus of human 4-1BBL was used as an immunogen.

Molecular Weight: 26 kDA

Comments:

Background: 4-1BB was originally described as a cDNA expressed by activated murine T cells and subsequently demonstrated to encode a member of the tumor necrosis factor receptor family of integral membrane proteins. Recently, a murine ligand for 4-1BB (mu4-1BB-L) was cloned and demonstrated to be a member of an emerging family of ligands with structural homology to tumor necrosis factor. Both monoclonal antibody to hu4-1BB and cells transfected with hu4-1BB-L induced a strong proliferative response in mitogen co-stimulated primary T cells. In contrast, ligation of 4-1BB on T cell clones enhanced activation-induced cell death when triggered by engagement of the TCR/CD3 complex. (1). To reject tumors, T cells must overcome poor tumor immunogenicity and an adverse tumor microenvironment. It has been shown that primary human T cells expressing CD80 and 4-1BBL vigorously respond to tumor cells lacking costimulatory ligands and provoke potent rejection of large, systemic tumors in immunodeficient mice (2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:50 ICC: ~~ 1:100 IHC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: 4-1BBL Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Cell Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Alderson, Smith, Tough et al.: "Molecular and biological characterization of human 4-1BB and its ligand." in: European journal of immunology, Vol. 24, Issue 9, pp. 2219-27, 1994 (PubMed).

Stephan, Ponomarev, Brentjens et al.: "T cell-encoded CD80 and 4-1BBL induce auto- and transcostimulation, resulting in potent tumor rejection." in: Nature medicine, Vol. 13, Issue 12, pp. 1440-9, 2007 (PubMed).