anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1)

Details for Product anti-EIF4EBP1 Antibody No. ABIN649233
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Reactivity
Human
(448), (262), (203), (2), (1), (1), (1), (1)
Host
Rabbit
(429), (73), (25)
Clonality
Monoclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
(30), (26), (19), (15), (9), (9), (8), (8), (6), (6), (6), (6), (6), (6)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(341), (239), (217), (75), (60), (36), (27), (22), (18), (17), (16), (8), (6), (6), (4), (3), (1)
Pubmed 1 reference available
Quantity 100 μL
Options
Shipping to United States ( )
Availability Please contact our customer support.
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Specificity A synthetic peptide corresponding to residues near the C-term of human4E-BP1 was used as immunogen.
Alternative Name 4E-BP1 (EIF4EBP1 Antibody Abstract)
Background 4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will not (2). Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)
Synonyms: EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Molecular Weight 15-20 kDA
Gene ID 1978
UniProt Q13541
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Pathways PI3K-Akt Signaling Pathway
Application Notes IHC: = 1:50-100, WB: = 1:1000
Comment

Background: 4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will not (2). Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment 4E-BP1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Gingras, Kennedy, OLeary et al.: "4E-BP1, a repressor of mRNA translation, is phosphorylated and inactivated by the Akt(PKB) signaling pathway." in: Genes & development, Vol. 12, Issue 4, pp. 502-13, 1998 (PubMed).

Validation Images
Catalog No. ABIN649233
421.43 $
Plus shipping costs $45.00
Add to Basket

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)
Did you look for something else?