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Details for Product No. ABIN649233

Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) antibody

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Antigen
Synonyms
4E-BP1, 4EBP1, BP-1, PHAS-I, 4e-bp1, AA959816, 153432_at, 43-BP, 4E-BP, 4EBP, 4e-bp, 4eBP, 4ebp, BcDNA.HL08053, BcDNA:HL08053, CG8846, CT8705, Dm 4E-BP, Dmel\\CG8846, Phas1, Thor/EIF4EBP, anon-WO01727 ... show more
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
Pubmed 1 reference available
Catalog no. ABIN649233
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues near the C-term of human4E-BP1 was used as immunogen.
Alternative Name 4E-BP1
Background 4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will not (2). Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)
Synonyms: EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Molecular Weight 15-20 kDA
Gene ID 1978
UniProt Q13541
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:50 FC: ~~ 1:20 IHC: ~~ 1:50~100
Comment

Background: 4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will not (2). Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment 4E-BP1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Gingras, Kennedy, OLeary et al.: "4E-BP1, a repressor of mRNA translation, is phosphorylated and inactivated by the Akt(PKB) signaling pathway." in: Genes & development, Vol. 12, Issue 4, pp. 502-13, 1998 (PubMed).

Alternatives for antigen "Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)", type "Antibodies"
Hosts (302), (40), (21)
Reactivities (308), (234), (182), (60), (60), (38), (24), (1)
Applications (244), (139), (122), (87), (69), (31), (20), (17), (9), (6), (6), (4), (2), (1), (1)
Conjugates (20), (13), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6)
Epitopes (33), (23), (21), (18), (17), (17), (16), (13), (13), (12), (7), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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