Acyl-CoA Dehydrogenase, C-4 To C-12 Straight Chain (ACADM) antibody

Antigen: Acyl-CoA Dehydrogenase, C-4 To C-12 Straight Chain (ACADM)

Synonyms: acaDM, MCAD, ACAD1, MCADH, FLJ18227, FLJ93013, FLJ99884, ACADM, acadm, MGC78996, MGC128274

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649241

Additional Information

Alternative name: ACADM

Gene ID: 10232

UniProt: P11310

Characteristics: Rabbit Monoclonal IgG

Specificity: A synthetic peptide corresponding to residues in human ACADM was used as an immunogen.

Molecular Weight: 43 kDA

Comments:

Background: Medium-chain specific acyl-CoA dehydrogenase (ACADM) is a homotetramer enzyme of the mitochondrial flavoenzyme family that catalyzes the initial reaction in medium chain fatty acid beta-oxidation (1, 2). ACADM is specific for acyl chain lengths of 4 to 16 (3). It utilizes the electron transfer flavoprotein (ETF) as an electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (3). Defects in the ACADM gene cause medium-chain acyl-CoA dehydrogenase deficiency (MCADD), a metabolic disorder characterized by fasting hypoglycemia (3). Mutation analysis can diagnose MCADD, which is currently screened in newborns, since its monogene may be modified by genetic variations (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10~100 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: ACADM Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Zhang, Kelly, Kim et al.: "Structural organization and regulatory regions of the human medium-chain acyl-CoA dehydrogenase gene." in: Biochemistry, Vol. 31, Issue 1, pp. 81-9, 1992 (PubMed).

Gregersen, Andresen, Corydon et al.: "Mutation analysis in mitochondrial fatty acid oxidation defects: Exemplified by acyl-CoA dehydrogenase deficiencies, with special focus on genotype-phenotype relationship." in: Human mutation, Vol. 18, Issue 3, pp. 169-89, 2001 (PubMed).

"The Universal Protein Resource (UniProt) 2009." in: Nucleic acids research, Vol. 37, Issue Database issue, pp. D169-74, 2008 (PubMed).