Acetyl-CoA Carboxylase alpha (ACACA) antibody

Antigen: Acetyl-CoA Carboxylase alpha (ACACA)

Synonyms: ACC, ACAC, ACC1, ACCA

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649246

Additional Information

Alternative name: Acetyl-CoA1 (ACC1)

Gene ID: 31

UniProt: Q13085

Description: Other names: ACACA, ACAC, ACC1, ACCA, Biotin carboxylase, ACC-alpha

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in the human Acetyl-CoA carboxylase 1 was used as an immunogen.

Molecular Weight: 265 kDA

Comments:

Background: Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500~2000 IHC: ~~ 1:50~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Acetyl-CoA1 (ACC1) Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Wakil, Stoops, Joshi: "Fatty acid synthesis and its regulation." in: Annual review of biochemistry, Vol. 52, pp. 537-79, 1983 (PubMed).

Abu-Elheiga, Matzuk, Abo-Hashema et al.: "Continuous fatty acid oxidation and reduced fat storage in mice lacking acetyl-CoA carboxylase 2." in: Science (New York, N.Y.), Vol. 291, Issue 5513, pp. 2613-6, 2001 (PubMed).

Mao, Chirala, Wakil: "Human acetyl-CoA carboxylase 1 gene: presence of three promoters and heterogeneity at the 5'-untranslated mRNA region." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 13, pp. 7515-20, 2003 (PubMed).