Cholinergic Receptor, Nicotinic, beta Polypeptide 1 (Muscle) (CHRNB1) (Extracellular) antibody

Details for Product No. ABIN649249
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Antigen
Epitope
Extracellular
(1)
Reactivity
Human, Rat (Rattus)
(3), (1)
Host
Rabbit
(3)
Clonality
Monoclonal
Application
Western Blotting (WB)
(3), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 μL
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Catalog No. ABIN649249
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Specificity A synthetic peptide corresponding to residues in the extracellular region of human Acetylcholine receptor subunit beta was used as an immunogen.
Alternative Name ACHR-beta
Background The nicotinic acetylcholine receptor controls electrical signaling between nerve and muscle cells by opening and closing a gated, membrane-spanning pore. The gate is a constricting hydrophobic girdle at the middle of the lipid bi-layer, formed by weak interactions between neighboring inner helices. When acetylcholine enters the ligand-binding domain, it triggers rotations of the protein chains on opposite sides of the entrance to the pore (1). The widespread distribution of the beta subunit suggests it may serve as a common subunit in different AChR combinations in various brain regions. Regulation of the expression of beta subunit appears to be relatively unrestrained, with an apparent excess of protein synthesized in the cytoplasm relative to that which ultimately arrives at functional targets in the plasma membrane (2).
Synonyms: CHRNB1, ACHRB, CHRNB, Acetylcholine receptor subunit beta
Molecular Weight 56 kDA
Gene ID 1140
UniProt P11230
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Neurology
Application Notes The suggested dilution is: WB: = 1:5000
Comment

Background: The nicotinic acetylcholine receptor controls electrical signaling between nerve and muscle cells by opening and closing a gated, membrane-spanning pore. The gate is a constricting hydrophobic girdle at the middle of the lipid bi-layer, formed by weak interactions between neighboring inner helices. When acetylcholine enters the ligand-binding domain, it triggers rotations of the protein chains on opposite sides of the entrance to the pore (1). The widespread distribution of the beta subunit suggests it may serve as a common subunit in different AChR combinations in various brain regions. Regulation of the expression of beta subunit appears to be relatively unrestrained, with an apparent excess of protein synthesized in the cytoplasm relative to that which ultimately arrives at functional targets in the plasma membrane (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment ACHR-beta Antibody (extracellular) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Miyazawa, Fujiyoshi, Unwin: "Structure and gating mechanism of the acetylcholine receptor pore." in: Nature, Vol. 423, Issue 6943, pp. 949-55, 2003 (PubMed).

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