|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
|4 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: ACTC1, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues near the N-terminus of human Actin was used as an immunogen. This Actin antibody recognizes all muscle actins.|
|Molecular Weight||42 kDA|
Background: Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).
|Synonyms||A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051|
|Application Notes||The suggested dilution is: WB: ~~ 1:10000~20000 IP: ~~ 1:40 FC: ~~ 1:20 ICC: ~~ 1:250~500 IHC: ~~ 1:100~200|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Actin Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Signaling, Protein Modifications, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Herman: "Actin isoforms." in: Current opinion in cell biology, Vol. 5, Issue 1, pp. 48-55, 1993 (PubMed).
Olson, Michels, Thibodeau et al.: "Actin mutations in dilated cardiomyopathy, a heritable form of heart failure." in: Science (New York, N.Y.), Vol. 280, Issue 5364, pp. 750-2, 1998 (PubMed).
Bailly, Ichetovkin, Grant et al.: "The F-actin side binding activity of the Arp2/3 complex is essential for actin nucleation and lamellipod extension." in: Current biology : CB, Vol. 11, Issue 8, pp. 620-5, 2001 (PubMed).
Lim, Wong, Ooi et al.: "Selective tyrosine hyperphosphorylation of cytoskeletal and stress proteins in primary human breast cancers: implications for adjuvant use of kinase-inhibitory drugs." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 12 Pt 1, pp. 3980-7, 2004 (PubMed).