Actin antibody

Details for Product No. ABIN649250
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Antigen
Synonyms
Act57, Act57A, Act57b, Actin, BEST:RE38067, C, CG10067, CG30294, Dmel\\CG10067, M, T10, act 57A, act57A, act57B, act57b, actin, anon-EST:CL2c12, 143060_f_at, CG7478, D, Dmel\\CG7478, act 79B, act79B,  ... show more
Act57, Act57A, Act57b, Actin, BEST:RE38067, C, CG10067, CG30294, Dmel\\CG10067, M, T10, act 57A, act57A, act57B, act57b, actin, anon-EST:CL2c12, 143060_f_at, CG7478, D, Dmel\\CG7478, act 79B, act79B, mus79B, Act(88F), Act88-F, Act88f, CG5178, Dmel\\CG5178, E, Ifm(3)7, act 88F, act88F, rsd, Act87e, CG18290, Dmel\\CG18290, act 87E, act87E, mus87E, Act88F, Dsim\\GD19025, GD19025, dsim_GLEANR_2829, 42A, A, AFFX-Dros-ACTIN_M_r_at, Act, Act42, Act42a, Actin/BAP47, BAP47, CG12051, Dmel\\CG12051, act 42A, act42A, cytoplasmic show less
Reactivity
Human, Rat (Rattus)
(107), (72), (66), (44), (21), (15), (13), (12), (8), (7), (6), (6), (6), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host
Rabbit
(91), (65), (4)
Clonality
Monoclonal
Conjugate
Un-conjugated
(9), (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(109), (45), (43), (42), (32), (28), (26), (20), (17), (2), (1), (1)
Pubmed 4 references available
Quantity 100 µL
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Catalog No. ABIN649250
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Specificity A synthetic peptide corresponding to residues near the N-terminus of human Actin was used as an immunogen. This Actin antibody recognizes all muscle actins.
Background Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).
Synonyms: ACTC1, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin
Molecular Weight 42 kDA
Gene ID 70
UniProt P68032
Research Area Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:10000-20000 , IHC: = 1:100-200
Comment

Background: Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Actin Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Herman: "Actin isoforms." in: Current opinion in cell biology, Vol. 5, Issue 1, pp. 48-55, 1993 (PubMed).

Olson, Michels, Thibodeau et al.: "Actin mutations in dilated cardiomyopathy, a heritable form of heart failure." in: Science (New York, N.Y.), Vol. 280, Issue 5364, pp. 750-2, 1998 (PubMed).

Bailly, Ichetovkin, Grant et al.: "The F-actin side binding activity of the Arp2/3 complex is essential for actin nucleation and lamellipod extension." in: Current biology : CB, Vol. 11, Issue 8, pp. 620-5, 2001 (PubMed).

Lim, Wong, Ooi et al.: "Selective tyrosine hyperphosphorylation of cytoskeletal and stress proteins in primary human breast cancers: implications for adjuvant use of kinase-inhibitory drugs." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 12 Pt 1, pp. 3980-7, 2004 (PubMed).

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