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Adducin alpha (C-Term) antibody

Details for Product No. ABIN649254
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Antigen
Synonyms ADDA, AI256389
Epitope
C-Term
(3), (1), (1), (1)
Reactivity
Human
(6), (5), (4)
Host
Rabbit
(6)
Clonality
Monoclonal
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(5), (4), (2)
Pubmed 3 references available
Quantity 100 µL
Options
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Catalog No. ABIN649254
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Specificity A synthetic peptide corresponding to residues in the C-term of human Adducin was used as immunogen.
Background Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits, α, and Beta, or α, and γ, subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha (5).
Synonyms: ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha
Molecular Weight 130 kDA
Gene ID 118
UniProt P35611
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:500-1000 , IHC: = 1:250-500
Comment

Background: Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits, α, and β,, or α, and γ, subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Adducin alpha Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Ling, Gardner, Bennett: "Protein kinase C phosphorylates a recently identified membrane skeleton-associated calmodulin-binding protein in human erythrocytes." in: The Journal of biological chemistry, Vol. 261, Issue 30, pp. 13875-8, 1986 (PubMed).

Dong, Chapline, Mousseau et al.: "35H, a sequence isolated as a protein kinase C binding protein, is a novel member of the adducin family." in: The Journal of biological chemistry, Vol. 270, Issue 43, pp. 25534-40, 1995 (PubMed).

Kuhlman, Hughes, Bennett et al.: "A new function for adducin. Calcium/calmodulin-regulated capping of the barbed ends of actin filaments." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 7986-91, 1996 (PubMed).

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