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Details for Product No. ABIN649254

Adducin alpha (C-Term) antibody

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Antigen
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Pubmed 3 references available
Catalog no. ABIN649254
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues in the C-term of human Adducin was used as immunogen.
Background Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits, α, and Beta, or α, and γ, subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha (5).
Synonyms: ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha
Molecular Weight 130 kDA
Gene ID 118
UniProt P35611
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:500~1000 ICC: ~~ 1:250~500 IHC: ~~ 1:250~500
Comment

Background: Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits, α, and β,, or α, and γ, subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Adducin alpha Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Ling, Gardner, Bennett: "Protein kinase C phosphorylates a recently identified membrane skeleton-associated calmodulin-binding protein in human erythrocytes." in: The Journal of biological chemistry, Vol. 261, Issue 30, pp. 13875-8, 1986 (PubMed).

Dong, Chapline, Mousseau et al.: "35H, a sequence isolated as a protein kinase C binding protein, is a novel member of the adducin family." in: The Journal of biological chemistry, Vol. 270, Issue 43, pp. 25534-40, 1995 (PubMed).

Kuhlman, Hughes, Bennett et al.: "A new function for adducin. Calcium/calmodulin-regulated capping of the barbed ends of actin filaments." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 7986-91, 1996 (PubMed).

Alternatives for antigen "Adducin alpha", type "Antibodies"
Hosts (5)
Reactivities (5), (4), (3)
Applications (5), (3), (2), (1), (1)
Epitopes (3), (1), (1)
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